The Role of Temperature and Lipid Charge on Intake/Uptake of Cationic Gold Nanoparticles into Lipid Bilayers

Show full item record

Permalink

http://hdl.handle.net/10138/305908

Citation

Lolicato , F , Joly , L , Martinez-Seara , H , Fragneto , G , Scoppola , E , Bombelli , F B , Vattulainen , I , Akola , J & Maccarini , M 2019 , ' The Role of Temperature and Lipid Charge on Intake/Uptake of Cationic Gold Nanoparticles into Lipid Bilayers ' , Small , vol. 15 , no. 23 , 1805046 . https://doi.org/10.1002/smll.201805046

Title: The Role of Temperature and Lipid Charge on Intake/Uptake of Cationic Gold Nanoparticles into Lipid Bilayers
Author: Lolicato, Fabio; Joly, Loic; Martinez-Seara, Hector; Fragneto, Giovanna; Scoppola, Ernesto; Bombelli, Francesca Baldelli; Vattulainen, Ilpo; Akola, Jaakko; Maccarini, Marco
Contributor: University of Helsinki, Department of Physics
University of Helsinki, Department of Physics
Date: 2019-06
Number of pages: 15
Belongs to series: Small
ISSN: 1613-6810
URI: http://hdl.handle.net/10138/305908
Abstract: Understanding the molecular mechanisms governing nanoparticle-membrane interactions is of prime importance for drug delivery and biomedical applications. Neutron reflectometry (NR) experiments are combined with atomistic and coarse-grained molecular dynamics (MD) simulations to study the interaction between cationic gold nanoparticles (AuNPs) and model lipid membranes composed of a mixture of zwitterionic di-stearoyl-phosphatidylcholine (DSPC) and anionic di-stearoyl-phosphatidylglycerol (DSPG). MD simulations show that the interaction between AuNPs and a pure DSPC lipid bilayer is modulated by a free energy barrier. This can be overcome by increasing temperature, which promotes an irreversible AuNP incorporation into the lipid bilayer. NR experiments confirm the encapsulation of the AuNPs within the lipid bilayer at temperatures around 55 degrees C. In contrast, the AuNP adsorption is weak and impaired by heating for a DSPC-DSPG (3:1) lipid bilayer. These results demonstrate that both the lipid charge and the temperature play pivotal roles in AuNP-membrane interactions. Furthermore, NR experiments indicate that the (negative) DSPG lipids are associated with lipid extraction upon AuNP adsorption, which is confirmed by coarse-grained MD simulations as a lipid-crawling effect driving further AuNP aggregation. Overall, the obtained detailed molecular view of the interaction mechanisms sheds light on AuNP incorporation and membrane destabilization.
Subject: gold nanoparticles
lipid membranes
molecular dynamics simulations
nanotoxicity
neutron reflectometry
MOLECULAR-DYNAMICS
SPECULAR REFLECTION
AU NANOPARTICLES
SURFACE
MEMBRANES
SIZE
RECOGNITION
PERMEATION
MONOLAYERS
CHEMISTRY
114 Physical sciences
Rights:


Files in this item

Total number of downloads: Loading...

Files Size Format View
Lolicato_et_al_2019_Small.pdf 4.409Mb PDF View/Open

This item appears in the following Collection(s)

Show full item record