Presence of Stromal Cells Enhances Epithelial-to-Mesenchymal Transition (EMT) Induction in Lung Bronchial Epithelium after Protracted Exposure to Oxidative Stress of Gamma Radiation

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dc.contributor.author Acheva, Anna
dc.contributor.author Haghdoost, Siamak
dc.contributor.author Sollazzo, Alice
dc.contributor.author Launonen, Virpi
dc.contributor.author Kämäräinen, Meerit
dc.date.accessioned 2019-10-10T10:52:02Z
dc.date.available 2019-10-10T10:52:02Z
dc.date.issued 2019-09-08
dc.identifier.citation Acheva , A , Haghdoost , S , Sollazzo , A , Launonen , V & Kämäräinen , M 2019 , ' Presence of Stromal Cells Enhances Epithelial-to-Mesenchymal Transition (EMT) Induction in Lung Bronchial Epithelium after Protracted Exposure to Oxidative Stress of Gamma Radiation ' , Oxidative medicine and cellular longevity , vol. 2019 , 4120379 . https://doi.org/10.1155/2019/4120379
dc.identifier.other PURE: 127354624
dc.identifier.other PURE UUID: 5afcb4a5-1f73-44e4-9b81-cf0d4922a13d
dc.identifier.other WOS: 000487809200002
dc.identifier.other ORCID: /0000-0001-9447-8977/work/71907273
dc.identifier.uri http://hdl.handle.net/10138/305932
dc.description.abstract The aim of the study was to investigate the role of a microenvironment in the induction of epithelial-to-mesenchymal transition (EMT) as a sign of early stages of carcinogenesis in human lung epithelial cell lines after protracted low-dose rate gamma-radiation exposures. BEAS-2B and HBEC-3KT lung cell lines were irradiated with low-dose rate gamma-rays (Cs-137, 1.4 or 14 mGy/h) to 0.1 or 1 Gy with or without adding TGF-beta. TGF-beta-treated samples were applied as positive EMT controls and tested in parallel to find out if the radiation has a potentiating effect on the EMT induction. To evaluate the effect of the stromal component, the epithelial cells were irradiated in cocultures with stromal MRC-9 lung fibroblasts. On day 3 post treatment, the EMT markers: alpha-SMA, vimentin, fibronectin, and E-cadherin, were analyzed. The oxidative stress levels were evaluated by 8-oxo-dG analysis in both epithelial and fibroblast cells. The protracted exposure to low Linear Energy Transfer (LET) radiation at the total absorbed dose of 1 Gy was able to induce changes suggestive of EMT. The results show that the presence of the stromal component and its signaling (TGF-beta) in the cocultures enhances the EMT. Radiation had a minor cumulative effect on the TGF-beta-induced EMT with both doses. The oxidative stress levels were higher than the background in both epithelial and stromal cells post chronic irradiation (0.1 and 1 Gy); as for the BEAS-2B cell line, the increase was statistically significant. We suggest that the induction of EMT in bronchial epithelial cells by radiation requires more than single acute exposure and the presence of stromal component might enhance the effect through free radical production and accumulation. en
dc.format.extent 14
dc.language.iso eng
dc.relation.ispartof Oxidative medicine and cellular longevity
dc.rights cc_by
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject CANCER-ASSOCIATED FIBROBLASTS
dc.subject TGF-BETA
dc.subject NUCLEOTIDE POOL
dc.subject TUMOR-GROWTH
dc.subject ACTIVATION
dc.subject MICROENVIRONMENT
dc.subject CARCINOGENESIS
dc.subject MECHANISMS
dc.subject 8-OXO-DG
dc.subject TARGET
dc.subject 1182 Biochemistry, cell and molecular biology
dc.title Presence of Stromal Cells Enhances Epithelial-to-Mesenchymal Transition (EMT) Induction in Lung Bronchial Epithelium after Protracted Exposure to Oxidative Stress of Gamma Radiation en
dc.type Article
dc.contributor.organization Veterinary Pathology and Parasitology
dc.contributor.organization Soluvälitteiset voimat syöpäsolujen invaasiossa
dc.contributor.organization Veterinary Biosciences
dc.contributor.organization Faculty of Veterinary Medicine
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.1155/2019/4120379
dc.relation.issn 1942-0900
dc.rights.accesslevel openAccess
dc.type.version publishedVersion

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