Direct Estimation of HDL-Mediated Cholesterol Efflux Capacity from Serum

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Kuusisto , S , Holmes , M , Ohukainen , P , Kangas , A J , Karsikas , M , Tiainen , M , Perola , M , Salomaa , V , Kettunen , J & Ala-Korpela , M 2019 , ' Direct Estimation of HDL-Mediated Cholesterol Efflux Capacity from Serum ' , Clinical Chemistry (Washington, DC) , vol. 65 , no. 8 , pp. 1042-1050 .

Title: Direct Estimation of HDL-Mediated Cholesterol Efflux Capacity from Serum
Author: Kuusisto, Sanna; Holmes, Michael; Ohukainen, Pauli; Kangas, Antti J.; Karsikas, Mari; Tiainen, Mika; Perola, Markus; Salomaa, Veikko; Kettunen, Johannes; Ala-Korpela, Mika
Contributor: University of Helsinki, University Management
Date: 2019-08
Language: eng
Number of pages: 9
Belongs to series: Clinical Chemistry (Washington, DC)
ISSN: 0009-9147
Abstract: BACKGROUND: HDL-mediated cholesterol efflux capacity (HDL-CEC) is a functional attribute that may have a protective role in atherogenesis. However, the estimation of HDL-CEC is based on in vitro cell assays that are laborious and hamper large-scale phenotyping. METHODS: Here, we present a cost-effective high-throughput nuclear magnetic resonance (NMR) spectroscopy method to estimate HDL-CEC directly from serum. We applied the new method in a population-based study of 7603 individuals including 574 who developed incident coronary heart disease (CHD) during 15 years of follow-up, making this the largest quantitative study for HDL-CEC. RESULTS: As estimated by NMR-spectroscopy, a 1-SD higher HDL-CEC was associated with a lower risk of incident CHD (hazards ratio, 0.86; 95% CI, 0.79-0.93, adjusted for traditional risk factors and HDL-C). These findings are consistent with published associations based on in vitro cell assays. CONCLUSIONS: These corroborative large-scale findings provide further support for a potential protective role of HDL-CEC in CHD and substantiate this new method and its future applications. (C) 2019 American Association for Clinical Chemistry
3111 Biomedicine

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