Molecular forms and fragments of salivary MMP-8 in relation to periodontitis

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http://hdl.handle.net/10138/306204

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Gürsoy , U K , Könönen , E , Tervahartiala , T , Gürsoy , M , Pitkänen , J , Torvi , P , Suominen , A-L , Pussinen , P & Sorsa , T 2018 , ' Molecular forms and fragments of salivary MMP-8 in relation to periodontitis ' , Journal of Clinical Periodontology , vol. 45 , no. 12 , pp. 1421-1428 . https://doi.org/10.1111/jcpe.13024

Title: Molecular forms and fragments of salivary MMP-8 in relation to periodontitis
Author: Gürsoy, Ulvi K.; Könönen, Eija; Tervahartiala, Taina; Gürsoy, Mervi; Pitkänen, Jari; Torvi, Paula; Suominen, Anna-Liisa; Pussinen, Pirkko; Sorsa, Timo
Contributor: University of Helsinki, Clinicum
University of Helsinki, Department of Oral and Maxillofacial Diseases
University of Helsinki, Timo Sorsa / Principal Investigator
Date: 2018-12
Language: eng
Number of pages: 8
Belongs to series: Journal of Clinical Periodontology
ISSN: 0303-6979
URI: http://hdl.handle.net/10138/306204
Abstract: Aim To investigate the molecular forms of salivary matrix metalloproteinase (MMP)-8 in relation to periodontitis. Materials and Methods Molecular forms, degree of activation and fragmentation of neutrophilic and mesenchymal-type MMP-8 isoforms were analysed from salivary samples of 81 subjects with generalized periodontitis, 63 subjects with localized periodontitis and 79 subjects without pocket teeth, by using western-immunoblots with computer quantitation. In addition, human recombinant proMMP-8 was in vitro activated by Treponema denticola chymotrypsin-like protease (Td-CTLP), sodium hypochlorite (NaOCl, 1 mM, oxidant) or amino phenyl mercuric acetate (APMA, 1 mM). Results In saliva of periodontitis-affected individuals, MMP-8 is found in multiple forms, that is, complexes, active and pro-forms of neutrophilic and mesenchymal-type MMP-8, and especially 20-27 kDa fragments. The quantity of these fragments was elevated in both localized and generalized forms of periodontitis. Moreover, the tested activators (Td-CTLP, NaOCl and APMA) activated inactive proMMP-8, resulting in fragments of 20-27 kDa, in vitro, and salivary concentrations of T. denticola correlated significantly with salivary levels of fragmented MMP-8. Conclusion The present results indicate that during the development and progression of periodontitis, MMP-8 appears as activated and fragmented, and treponemal proteases most likely play role in this cascade.
Subject: collagenase
neutrophil
periodontal disease
saliva
HUMAN NEUTROPHIL COLLAGENASE
GINGIVAL CREVICULAR FLUID
LATENT HUMAN NEUTROPHIL
MATRIX METALLOPROTEINASES
PORPHYROMONAS-GINGIVALIS
ACTIVATION
RELEASE
EXPRESSION
PROTEASES
MARKERS
313 Dentistry
3126 Surgery, anesthesiology, intensive care, radiology
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