Development of omics-based protocols for the microbiological characterization of multi-strain formulations marketed as probiotics : the case of VSL#3

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Mora , D , Filardi , R , Arioli , S , Boeren , S , Aalvink , S & de Vos , W M 2019 , ' Development of omics-based protocols for the microbiological characterization of multi-strain formulations marketed as probiotics : the case of VSL#3 ' , Microbial Biotechnology , vol. 12 , no. 6 , pp. 1371-1386 . https://doi.org/10.1111/1751-7915.13476

Title: Development of omics-based protocols for the microbiological characterization of multi-strain formulations marketed as probiotics : the case of VSL#3
Author: Mora, Diego; Filardi, Rossella; Arioli, Stefania; Boeren, Sjef; Aalvink, Steven; de Vos, Willem M.
Contributor: University of Helsinki, Director and Common Matters
Date: 2019-11
Language: eng
Number of pages: 16
Belongs to series: Microbial Biotechnology
ISSN: 1751-7915
URI: http://hdl.handle.net/10138/306363
Abstract: The growing commercial interest in multi-strain formulations marketed as probiotics has not been accompanied by an equal increase in the evaluation of quality levels of these biotechnological products. The multi-strain product VSL#3 was used as a model to setup a microbiological characterization that could be extended to other formulations with high complexity. Shotgun metagenomics by deep Illumina sequencing was applied to DNA isolated from the commercial VSL#3 product to confirm strains identity safety and composition. Single-cell analysis was used to evaluate the cell viability, and beta-galactosidase and urease activity have been used as marker to monitor the reproducibility of the production process. Similarly, these lots were characterized in detail by a metaproteomics approach for which a robust protein extraction protocol was combined with advanced mass spectrometry. The results identified over 1600 protein groups belonging to all strains present in the VSL#3 formulation. Of interest, only 3.2 % proteins showed significant differences mainly related to small variations in strain abundance. The protocols developed in this study addressed several quality criteria that are relevant for marketed multi-strain products and these represent the first efforts to define the quality of complex probiotic formulations such as VSL#3.
Subject: ANIMALIS SUBSP LACTIS
COMPLETE GENOME SEQUENCE
FLOW-CYTOMETRY
STREPTOCOCCUS-THERMOPHILUS
HYDROGEN-PEROXIDE
PRODUCTS
BACTERIA
REVEALS
QUALITY
MILK
3111 Biomedicine
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