PIM-related kinases selectively regulate olfactory sensations in C. elegans

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dc.contributor.author Kalichamy, Karunambigai S.
dc.contributor.author Ikkala, Kaisa
dc.contributor.author Pörsti, Jonna
dc.contributor.author Santio, Niina M.
dc.contributor.author Tuomaala, Joel
dc.contributor.author Jha, Sweta
dc.contributor.author Holmberg, Carina
dc.contributor.author Koskinen, Päivi J.
dc.date.accessioned 2019-10-29T10:05:03Z
dc.date.available 2019-10-29T10:05:03Z
dc.date.issued 2019
dc.identifier.citation Kalichamy , K S , Ikkala , K , Pörsti , J , Santio , N M , Tuomaala , J , Jha , S , Holmberg , C & Koskinen , P J 2019 , ' PIM-related kinases selectively regulate olfactory sensations in C. elegans ' , eNeuro , vol. 6 , no. 4 , UNSP ENEURO.0003-19.2019 . https://doi.org/10.1523/ENEURO.0003-19.2019
dc.identifier.other PURE: 127722256
dc.identifier.other PURE UUID: 2d78a80a-a9a4-45c4-b71c-bd827e858269
dc.identifier.other WOS: 000483309700002
dc.identifier.other ORCID: /0000-0003-1990-8206/work/64001764
dc.identifier.other ORCID: /0000-0003-2836-4036/work/64002238
dc.identifier.uri http://hdl.handle.net/10138/306487
dc.description.abstract The mammalian PIM family of serine/threonine kinases regulate several cellular functions, such as cell survival and motility. Since PIM expression is observed in sensory organs, such as olfactory epithelium, we now wanted to explore the physiological roles of PIM kinascs there. As our model organism, we used the Caenorhabditis elegans nematodes, which express two PIM-related kinases, PRK-1 and PRK-2. We demonstrated PRKs to be true PIM orthologs with similar substrate specificity as well as sensitivity to PIM-inhibitory compounds. When we analysed the effects of pan-PIM inhibitors on C. elegans sensory functions, we observed that PRK activity is selectively required to support olfactory sensations to volatile repellents and attractants sensed by AWB and AWC(ON) neurons, respectively, but is dispensable for gustatory sensations. Analyses of prk-deficient mutant strains confirmed these findings and suggested that PRK-1, but not PRK-2 is responsible for the observed effects on olfaction. This regulatory role of PRK-1 is further supported by its observed expression in the head and tail neurons, including AWB and AWC neurons. Based on the evolutionary conservation of NM-related kinases, our data may have implications in regulation of also mammalian olfaction. en
dc.format.extent 42
dc.language.iso eng
dc.relation.ispartof eNeuro
dc.rights cc_by
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject EXPRESSION
dc.subject PROTEIN
dc.subject CELLS
dc.subject BAD
dc.subject 3112 Neurosciences
dc.subject 3124 Neurology and psychiatry
dc.subject 3111 Biomedicine
dc.title PIM-related kinases selectively regulate olfactory sensations in C. elegans en
dc.type Article
dc.contributor.organization CAN-PRO - Translational Cancer Medicine Program
dc.contributor.organization Research Programs Unit
dc.contributor.organization University of Helsinki
dc.contributor.organization University Management
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.1523/ENEURO.0003-19.2019
dc.relation.issn 2373-2822
dc.rights.accesslevel openAccess
dc.type.version publishedVersion

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