Graft glycocalyx degradation in human liver transplantation

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Passov , A , Schramko , A , Mäkisalo , H , Nordin , A , Andersson , S , Pesonen , E & Ilmakunnas , M 2019 , ' Graft glycocalyx degradation in human liver transplantation ' , PLoS One , vol. 14 , no. 8 , 0221010 . https://doi.org/10.1371/journal.pone.0221010

Title: Graft glycocalyx degradation in human liver transplantation
Author: Passov, Arie; Schramko, Alexey; Mäkisalo, Heikki; Nordin, Arno; Andersson, Sture; Pesonen, Eero; Ilmakunnas, Minna
Contributor: University of Helsinki, HUS Perioperative, Intensive Care and Pain Medicine
University of Helsinki, HUS Perioperative, Intensive Care and Pain Medicine
University of Helsinki, HUS Abdominal Center
University of Helsinki, HUS Abdominal Center
University of Helsinki, HUS Children and Adolescents
University of Helsinki, HUS Perioperative, Intensive Care and Pain Medicine
University of Helsinki, HUS Perioperative, Intensive Care and Pain Medicine
Date: 2019-08-15
Language: eng
Number of pages: 13
Belongs to series: PLoS One
ISSN: 1932-6203
URI: http://hdl.handle.net/10138/306585
Abstract: Objective Ischaemia/reperfusion-injury degrades endothelial glycocalyx. Graft glycocalyx degradation was studied in human liver transplantation. Methods To assess changes within the graft, blood was drawn from portal and hepatic veins in addition to systemic samples in 10 patients. Plasma syndecan-1, heparan sulfate and chondroitin sulfate, were measured with enzyme-linked immunosorbent assay. Results During reperfusion, syndecan-1 levels were higher in graft caval effluent [3118 (934–6141) ng/ml, P = 0.005] than in portal venous blood [101 (75–121) ng/ml], indicating syndecan-1 release from the graft. Concomitantly, heparan sulfate levels were lower in graft caval effluent [96 (32–129) ng/ml, P = 0.037] than in portal venous blood [112 (98–128) ng/ml], indicating heparan sulfate uptake within the graft. Chondroitin sulfate levels were equal in portal and hepatic venous blood. After reperfusion arterial syndecan-1 levels increased 17-fold (P <0.001) and heparan sulfate decreased to a third (P <0.001) towards the end of surgery. Conclusion Syndecan-1 washout from the liver indicates extensive glycocalyx degradation within the graft during reperfusion. Surprisingly, heparan sulfate was taken up by the graft during reperfusion. Corroborating previous experimental reports, this suggests that endogenous heparan sulfate might be utilized within the graft in the repair of damaged glycocalyx.
Subject: 3126 Surgery, anesthesiology, intensive care, radiology
EARLY ALLOGRAFT DYSFUNCTION
ENDOTHELIAL GLYCOCALYX
TRANEXAMIC ACID
SYNDECAN-1
SHOCK
MECHANISMS
PRESERVES
SURGERY
TRAUMA
INJURY
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