Immunoassay for serodiagnosis of Zika virus infection based on time-resolved Förster resonance energy transfer

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Kareinen , L , Hepojoki , S , Huhtamo , E , Korhonen , E M , Schmidt-Chanasit , J , Hedman , K , Hepojoki , J & Vapalahti , O 2019 , ' Immunoassay for serodiagnosis of Zika virus infection based on time-resolved Förster resonance energy transfer ' , PLoS One , vol. 14 , no. 7 , 0219474 , pp. e0219474 . https://doi.org/10.1371/journal.pone.0219474

Title: Immunoassay for serodiagnosis of Zika virus infection based on time-resolved Förster resonance energy transfer
Author: Kareinen, Lauri; Hepojoki, Satu; Huhtamo, Eili; Korhonen, Essi M.; Schmidt-Chanasit, Jonas; Hedman, Klaus; Hepojoki, Jussi; Vapalahti, Olli
Contributor organization: Veterinary Biosciences
Department of Virology
University of Helsinki
Medicum
Viral Zoonosis Research Unit
Klaus Hedman / Principal Investigator
HUSLAB
Helsinki One Health (HOH)
University Management
Veterinary Microbiology and Epidemiology
Olli Pekka Vapalahti / Principal Investigator
Virus infections and immunity
Date: 2019-07-23
Language: eng
Number of pages: 13
Belongs to series: PLoS One
ISSN: 1932-6203
DOI: https://doi.org/10.1371/journal.pone.0219474
URI: http://hdl.handle.net/10138/306595
Abstract: Zika virus (ZIKV) is a mosquito-borne pathogen causing a febrile illness with arthralgia, conjunctivitis and rash. The complications include Guillain-Barré syndrome, congenital brain and other abnormalities and miscarriage. The serodiagnosis of ZIKV infection is hampered by cross-reactivity with other members of the Flavivirus family, notably dengue (DENV). This report describes a novel serological platform for the diagnosis of ZIKV infection. The approach utilizes time-resolved Förster resonance energy transfer (TR-FRET) elicited by two chromophore-labeled proteins (a ZIKV antigen and a super-antigen) simultaneously binding to a given antibody molecule. The antigen used in the assay is ZIKV non-structural protein 1 (NS1) and the super-antigen is bacterial protein L. Three assay variants were developed: the first measuring all anti-ZIKV-NS1 antibodies (LFRET), the second measuring IgM and IgA (acute-LFRET) and the third measuring IgG (immunity-LFRET). The assays were evaluated with a panel of samples from clinical ZIKV cases in travelers (n = 25) and seronegative (n = 24) samples. DENV (n = 38), yellow fever (n = 16) and tick-borne-encephalitis (n = 20) seropositive samples were examined for assessment of flavivirus cross-reactivity. The diagnostic sensitivities of the respective LFRET assays were 92%, 100% and 83%, and the diagnostic specificities 88%, 95% and 100% for LFRET, acute-LFRET and immunity-LFRET. Furthermore, we evaluated the assays against a widely-used commercial ELISA. In conclusion, the new FRET-based serological approaches based on NS1 protein are applicable to diagnosing zika virus infections in travelers and differentiating them from other flavivirus infections.
Subject: OUTBREAK
PROTEIN
IGM
3111 Biomedicine
1183 Plant biology, microbiology, virology
Peer reviewed: Yes
Rights: cc_by
Usage restriction: openAccess
Self-archived version: publishedVersion


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