Immunoassay for serodiagnosis of Zika virus infection based on time-resolved Förster resonance energy transfer

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http://hdl.handle.net/10138/306595

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Kareinen , L , Hepojoki , S , Huhtamo , E , Korhonen , E M , Schmidt-Chanasit , J , Hedman , K , Hepojoki , J & Vapalahti , O 2019 , ' Immunoassay for serodiagnosis of Zika virus infection based on time-resolved Förster resonance energy transfer ' , PLoS One , vol. 14 , no. 7 , 0219474 , pp. e0219474 . https://doi.org/10.1371/journal.pone.0219474

Title: Immunoassay for serodiagnosis of Zika virus infection based on time-resolved Förster resonance energy transfer
Author: Kareinen, Lauri; Hepojoki, Satu; Huhtamo, Eili; Korhonen, Essi M.; Schmidt-Chanasit, Jonas; Hedman, Klaus; Hepojoki, Jussi; Vapalahti, Olli
Contributor: University of Helsinki, Veterinary Biosciences
University of Helsinki, Viral Zoonosis Research Unit
University of Helsinki, Viral Zoonosis Research Unit
University of Helsinki, Viral Zoonosis Research Unit
University of Helsinki, HUSLAB
University of Helsinki, Viral Zoonosis Research Unit
University of Helsinki, Helsinki One Health (HOH)
Date: 2019-07-23
Language: eng
Number of pages: 13
Belongs to series: PLoS One
ISSN: 1932-6203
URI: http://hdl.handle.net/10138/306595
Abstract: Zika virus (ZIKV) is a mosquito-borne pathogen causing a febrile illness with arthralgia, conjunctivitis and rash. The complications include Guillain-Barré syndrome, congenital brain and other abnormalities and miscarriage. The serodiagnosis of ZIKV infection is hampered by cross-reactivity with other members of the Flavivirus family, notably dengue (DENV). This report describes a novel serological platform for the diagnosis of ZIKV infection. The approach utilizes time-resolved Förster resonance energy transfer (TR-FRET) elicited by two chromophore-labeled proteins (a ZIKV antigen and a super-antigen) simultaneously binding to a given antibody molecule. The antigen used in the assay is ZIKV non-structural protein 1 (NS1) and the super-antigen is bacterial protein L. Three assay variants were developed: the first measuring all anti-ZIKV-NS1 antibodies (LFRET), the second measuring IgM and IgA (acute-LFRET) and the third measuring IgG (immunity-LFRET). The assays were evaluated with a panel of samples from clinical ZIKV cases in travelers (n = 25) and seronegative (n = 24) samples. DENV (n = 38), yellow fever (n = 16) and tick-borne-encephalitis (n = 20) seropositive samples were examined for assessment of flavivirus cross-reactivity. The diagnostic sensitivities of the respective LFRET assays were 92%, 100% and 83%, and the diagnostic specificities 88%, 95% and 100% for LFRET, acute-LFRET and immunity-LFRET. Furthermore, we evaluated the assays against a widely-used commercial ELISA. In conclusion, the new FRET-based serological approaches based on NS1 protein are applicable to diagnosing zika virus infections in travelers and differentiating them from other flavivirus infections.
Subject: OUTBREAK
PROTEIN
IGM
3111 Biomedicine
1183 Plant biology, microbiology, virology
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