Predicting tubal factor infertility by using markers of humoral and cell-mediated immune response against Chlamydia trachomatis

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Rantsi , T , Öhman , H , Puolakkainen , M , Bloigu , A , Paavonen , J , Surcel , H-M , Tiitinen , A & Joki-Korpela , P 2018 , ' Predicting tubal factor infertility by using markers of humoral and cell-mediated immune response against Chlamydia trachomatis ' , American Journal of Reproductive Immunology , vol. 80 , no. 5 , 13051 . https://doi.org/10.1111/aji.13051

Title: Predicting tubal factor infertility by using markers of humoral and cell-mediated immune response against Chlamydia trachomatis
Author: Rantsi, Tiina; Öhman, Hanna; Puolakkainen, Mirja; Bloigu, Aini; Paavonen, Jorma; Surcel, Heljä-Marja; Tiitinen, Aila; Joki-Korpela, Päivi
Contributor: University of Helsinki, Department of Obstetrics and Gynecology
University of Helsinki, Mirja Puolakkainen / Principal Investigator
University of Helsinki, Clinicum
University of Helsinki, Department of Obstetrics and Gynecology
University of Helsinki, Department of Obstetrics and Gynecology
Date: 2018-11
Language: eng
Number of pages: 7
Belongs to series: American Journal of Reproductive Immunology
ISSN: 1046-7408
URI: http://hdl.handle.net/10138/306623
Abstract: Problem: The accuracy of Chlamydia trachomatis antibody test in predicting tubal factor infertility (TFI) is limited, and more accurate methods are needed. Cell-mediated immune response (CMI) is crucial in the resolution of pathogen, but it may play an important role in the pathogenesis of C trachomatis-associated tubal damage. We studied whether combining the markers of C trachomatis-induced CMI to humoral immune response improves the accuracy of serology in TFI prediction. Method of study: Our prospective study consists of 258 subfertile women, of whom 22 (8.5%) had TFI. Women with other causes for subfertility served as a reference group. Serum C trachomatis major outer membrane protein (MOMP) and chlamydial heat-shock protein 60 (cHSP60) IgG antibodies were measured by ELISA. CMI was studied by lymphocyte proliferation assay in vitro. Results: Serological markers were more prevalent in women with TFI than in other subfertile women (40.9% vs 12.3% for MOMP IgG and 27.3% vs 10.2% for cHSP60 IgG). The best test combination for TFI was C. trachomatis MOMP and cHSP60 antibody with an accuracy of 90.3%, sensitivity of 22.7% and specificity of 96.6%. Positive post-test probability of this combination was 54.2%, and negative post-test probability was 12.4%. Adding of the markers of CMI did not significantly improve the accuracy of serology in TFI prediction. Conclusion: The accuracy of TFI prediction increases when the combination of C trachomatis MOMP and cHSP60 antibody tests is used. C trachomatis-induced CMI was common in our study population, but the markers of CMI did not predict TFI.
Subject: cell-mediated immune response
Chlamydia trachomatis
diagnostic test
serology
tubal evaluation
tubal factor infertility
HEAT-SHOCK-PROTEIN
SUBFERTILE WOMEN
ANTIBODY
METAANALYSIS
PREGNANCY
HYDROSALPINX
INFECTIONS
PREVALENCE
DIAGNOSIS
SEVERITY
3111 Biomedicine
3123 Gynaecology and paediatrics
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