Restructured Lactococcus lactis strains with emergent properties constructed by a novel highly efficient screening system

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dc.contributor.author Liu, Fulu
dc.contributor.author Zhang, Yating
dc.contributor.author Qiao, Wanjin
dc.contributor.author Zhu, Duolong
dc.contributor.author Xu, Haijin
dc.contributor.author Saris, Per E J
dc.contributor.author Qiao, Mingqiang
dc.date.accessioned 2019-11-18T11:37:22Z
dc.date.available 2019-11-18T11:37:22Z
dc.date.issued 2019-11-14
dc.identifier.citation Microbial Cell Factories. 2019 Nov 14;18(1):198
dc.identifier.uri http://hdl.handle.net/10138/307011
dc.description.abstract Abstract Background After 2.83% genome reduction in Lactococcus lactis NZ9000, a good candidate host for proteins production was obtained in our previous work. However, the gene deletion process was time consuming and laborious. Here, we proposed a convenient gene deletion method suitable for large-scale genome reduction in L. lactis NZ9000. Results Plasmid pNZ5417 containing a visually selectable marker PnisZ-lacZ was constructed, which allowed more efficient and convenient screening of gene deletion mutants. Using this plasmid, two large nonessential DNA regions, L-4A and L-5A, accounting for 1.25% of the chromosome were deleted stepwise in L. lactis 9k-3. When compared with the parent strain, the mutant L. lactis 9k-5A showed better growth characteristics, transformability, carbon metabolic capacity, and amino acids biosynthesis. Conclusions Thus, this study provides a convenient and efficient system for large-scale genome deletion in L. lactis through application of visually selectable marker, which could be helpful for rapid genome streamlining and generation of restructured L. lactis strains that can be used as cell factories.
dc.publisher BioMed Central
dc.subject Lactococcus lactis
dc.subject Visually selectable marker
dc.subject Large-scale genome deletion
dc.title Restructured Lactococcus lactis strains with emergent properties constructed by a novel highly efficient screening system
dc.date.updated 2019-11-18T11:37:22Z
dc.language.rfc3066 en
dc.rights.holder The Author(s)
dc.type.uri http://purl.org/eprint/entityType/ScholarlyWork
dc.type.uri http://purl.org/eprint/entityType/Expression
dc.type.uri http://purl.org/eprint/type/JournalArticle

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