Real-Time Label-Free Targeting Assessment and in Vitro Characterization of Curcumin-Loaded Poly-lactic-co-glycolic Acid Nanoparticles for Oral Colon Targeting

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Akl , M A , Kartal-Hodzic , A , Suutari , T , Oksanen , T , Montagner , I M , Rosato , A , Ismael , H R , Afouna , M I , Caliceti , P , Yliperttula , M , Samy , A M , Mastrotto , F , Salmaso , S & Viitala , T 2019 , ' Real-Time Label-Free Targeting Assessment and in Vitro Characterization of Curcumin-Loaded Poly-lactic-co-glycolic Acid Nanoparticles for Oral Colon Targeting ' , ACS Omega , vol. 4 , no. 16 , pp. 16878-16890 . https://doi.org/10.1021/acsomega.9b02086

Title: Real-Time Label-Free Targeting Assessment and in Vitro Characterization of Curcumin-Loaded Poly-lactic-co-glycolic Acid Nanoparticles for Oral Colon Targeting
Author: Akl, Mohamed A.; Kartal-Hodzic, Alma; Suutari, Teemu; Oksanen, Timo; Montagner, Isabella Monia; Rosato, Antonio; Ismael, Hatem R.; Afouna, Mohsen I.; Caliceti, Paolo; Yliperttula, Marjo; Samy, Ahmed M.; Mastrotto, Francesca; Salmaso, Stefano; Viitala, Tapani
Contributor organization: Division of Pharmaceutical Biosciences
Pharmaceutical biophysics group
Drug Research Program
Division of Pharmaceutical Chemistry and Technology
Biopharmaceutics Group
Date: 2019-10-01
Language: eng
Number of pages: 13
Belongs to series: ACS Omega
ISSN: 2470-1343
DOI: https://doi.org/10.1021/acsomega.9b02086
URI: http://hdl.handle.net/10138/307018
Abstract: The exploitation of curcumin for oral disease treatment is limited by its low solubility, poor bioavailability, and low stability. Surface-functionalized poly-lactic-co-glycolic acid (PLGA) nanoparticles (NPs) have shown promising results to ameliorate selective delivery of drugs to the gastro-intestinal tract. In this study, curcumin-loaded PLGA NPs (C-PLGA NPs) of about 200 nm were surface-coated with chitosan (CS) for gastro-intestinal mucosa adhesion, wheat germ agglutinin (WGA) for colon targeting or GE11 peptide for tumor colon targeting. Spectrometric and zeta potential analyses confirmed the successful functionalization of the C-PLGA NPs. Real-time label-free assessment of the cell membrane-NP interactions and NP cell uptake were performed by quartz crystal microbalance coupled with supported lipid bilayers and by surface plasmon resonance coupled with living cells. The study showed that CS-coated C-PLGA NPs interact with cells by the electrostatic mechanism, while both WGA- and GE11-coated C-PLGA NPs interact and are taken up by cells by specific active mechanisms. In vitro cell uptake studies corroborated the real-time label-free assessment by yielding a curcumin cell uptake of 7.3 ± 0.3, 13.5 ± 1.0, 27.3 ± 4.9, and 26.0 ± 1.3 μg per 104 HT-29 cells for noncoated, CS-, WGA-, and GE11-coated C-PLGA NPs, respectively. Finally, preliminary in vivo studies showed that the WGA-coated C-PLGA NPs efficiently accumulate in the colon after oral administration to healthy Balb/c mice. In summary, the WGA- and GE11-coated C-PLGA NPs displayed high potential for application as active targeted carriers for anticancer drug delivery to the colon.
Subject: 317 Pharmacy
BIODEGRADABLE POLYMERIC NANOPARTICLES
SURFACE-PLASMON RESONANCE
PLGA-BASED NANOPARTICLES
DRUG-DELIVERY
CHITOSAN
LECTIN
CELLS
PERSPECTIVES
FORMULATION
ANTICANCER
Peer reviewed: Yes
Rights: cc_by
Usage restriction: openAccess
Self-archived version: publishedVersion


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