How do cryptochromes and UVR8 interact in natural and simulated sunlight?

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Rai , N , Neugart , S , Yan , Y , Wang , F , Siipola , S M , Lindfors , A V , Winkler , J B , Albert , A , Brosche , M , Lehto , T , Morales , L O & Aphalo , P J 2019 , ' How do cryptochromes and UVR8 interact in natural and simulated sunlight? ' , Journal of Experimental Botany , vol. 70 , no. 18 , pp. 4975-4990 . https://doi.org/10.1093/jxb/erz236

Title: How do cryptochromes and UVR8 interact in natural and simulated sunlight?
Author: Rai, Neha; Neugart, Susanne; Yan, Yan; Wang, Fang; Siipola, Sari M.; Lindfors, Anders V.; Winkler, Jana Barbro; Albert, Andreas; Brosche, Mikael; Lehto, Tarja; Morales, Luis O.; Aphalo, Pedro J.
Contributor: University of Helsinki, Organismal and Evolutionary Biology Research Programme
University of Helsinki, Organismal and Evolutionary Biology Research Programme
University of Helsinki, Plant Biology
University of Helsinki, Organismal and Evolutionary Biology Research Programme
University of Helsinki, Plant Biology
University of Helsinki, Plant Biology
University of Helsinki, Plant Biology
Date: 2019-09-15
Language: eng
Number of pages: 16
Belongs to series: Journal of Experimental Botany
ISSN: 0022-0957
URI: http://hdl.handle.net/10138/307020
Abstract: Cryptochromes (CRYs) and UV RESISTANCE LOCUS 8 (UVR8) photoreceptors perceive UV-A/blue (315-500 nm) and UV-B (280-315 nm) radiation in plants, respectively. While the roles of CRYs and UVR8 have been studied in separate controlled-environment experiments, little is known about the interaction between these photoreceptors. Here, Arabidopsis wild-type Ler, CRYs and UVR8 photoreceptor mutants (uvr8-2, cry1cry2 and cry1cry2uvr8-2), and a flavonoid biosynthesis-defective mutant (tt4) were grown in a sun simulator. Plants were exposed to filtered radiation for 17 d or for 6 h, to study the effects of blue, UV-A, and UV-B radiation. Both CRYs and UVR8 independently enabled growth and survival of plants under solar levels of UV, while their joint absence was lethal under UV-B. CRYs mediated gene expression under blue light. UVR8 mediated gene expression under UV-B radiation, and in the absence of CRYs, also under UV-A. This negative regulation of UVR8-mediated gene expression by CRYs was also observed for UV-B. The accumulation of flavonoids was also consistent with this interaction between CRYs and UVR8. In conclusion, we provide evidence for an antagonistic interaction between CRYs and UVR8 and a role of UVR8 in UV-A perception.
Subject: Arabidopsis thaliana
blue light
cryptochromes
flavonoids
photoreceptor interaction
solar radiation
sun simulator
transcript abundance
ultraviolet radiation
UVR8
TRANSCRIPTION FACTOR HY5
OCCURRING FLAVONOID GLYCOSIDES
B-INDUCED PHOTOMORPHOGENESIS
SYNTHASE GENE-EXPRESSION
BLUE-LIGHT
SIGNAL-TRANSDUCTION
STRESS ACCLIMATION
ARABIDOPSIS
IDENTIFICATION
ACCUMULATION
1183 Plant biology, microbiology, virology
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