Do UV-A radiation and blue light during growth prime leaves to cope with acute high-light in photoreceptor mutants of Arabidopsis thaliana?

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Brelsford , C , Morales Suarez , L O , Nezval , J , Kotilainen , T K , Hartikainen , S M , Aphalo , P J & Robson , T M 2019 , ' Do UV-A radiation and blue light during growth prime leaves to cope with acute high-light in photoreceptor mutants of Arabidopsis thaliana? ' , Physiologia Plantarum , vol. 165 , no. 3 , pp. 537-554 . https://doi.org/10.1111/ppl.12749

Title: Do UV-A radiation and blue light during growth prime leaves to cope with acute high-light in photoreceptor mutants of Arabidopsis thaliana?
Author: Brelsford, Craig; Morales Suarez, Luis Orlando; Nezval, Jakub; Kotilainen, Titta Katariina; Hartikainen, Saara Maria; Aphalo, Pedro J.; Robson, Thomas Matthew
Contributor organization: Organismal and Evolutionary Biology Research Programme
Canopy Spectral Ecology and Ecophysiology
Viikki Plant Science Centre (ViPS)
Faculty of Biological and Environmental Sciences
Plant Biology
Plant stress and natural variation
Sensory and Physiological Ecology of Plants (SenPEP)
Date: 2019-03
Language: eng
Number of pages: 18
Belongs to series: Physiologia Plantarum
ISSN: 0031-9317
DOI: https://doi.org/10.1111/ppl.12749
URI: http://hdl.handle.net/10138/307034
Abstract: We studied how plants acclimated to growing conditions that included combinations of blue light (BL) and ultraviolet (UV)-A radiation, and whether their growing environment affected their photosynthetic capacity during and after a brief period of acute high light (as might happen during an under-canopy sunfleck). Arabidopsis thaliana Landsberg erecta wild-type were compared with mutants lacking functional blue light and UV photoreceptors: phototropin 1, cryptochromes (CRY1 and CRY2) and UV RESISTANT LOCUS 8 (uvr8). This was achieved using light-emitting-diode (LED) lamps in a controlled environment to create treatments with or without BL, in a split-plot design with or without UV-A radiation. We compared the accumulation of phenolic compounds under growth conditions and after exposure to 30 min of high light at the end of the experiment (46 days), and likewise measured the operational efficiency of photosystem II (phi PSII, a proxy for photosynthetic performance) and dark-adapted maximum quantum yield (F-v/F-m to assess PSII damage). Our results indicate that cryptochromes are the main photoreceptors regulating phenolic compound accumulation in response to BL and UV-A radiation, and a lack of functional cryptochromes impairs photosynthetic performance under high light. Our findings also reveal a role for UVR8 in accumulating flavonoids in response to a low UV-A dose. Interestingly, phototropin 1 partially mediated constitutive accumulation of phenolic compounds in the absence of BL. Low-irradiance BL and UV-A did not improve phi PSII and F-v/F-m upon our acute high-light treatment; however, CRYs played an important role in ameliorating high-light stress.
Subject: 1183 Plant biology, microbiology, virology
SYNTHASE GENE-EXPRESSION
PHOTOSYNTHETICALLY ACTIVE RADIATION
ULTRAVIOLET-B RADIATION
SIGNAL-TRANSDUCTION
PHOTOSYSTEM-II
HIGHER-PLANTS
CHLOROPHYLL FLUORESCENCE
ELECTRON-TRANSPORT
LEAF CHLOROPHYLL
RED-LIGHT
Peer reviewed: Yes
Rights: unspecified
Usage restriction: closedAccess
Self-archived version: submittedVersion


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