Fungal glucuronoyl esterases : Genome mining based enzyme discovery and biochemical characterization

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Dilokpimol , A , Makela , M R , Cerullo , G , Zhou , M , Varriale , S , Gidijala , L , Bras , J L A , Jutten , P , Piechot , A , Verhaert , R , Faraco , V , Hilden , K S & de Vries , R P 2018 , ' Fungal glucuronoyl esterases : Genome mining based enzyme discovery and biochemical characterization ' , New Biotechnology , vol. 40, Part B , pp. 282-287 . https://doi.org/10.1016/j.nbt.2017.10.003

Title: Fungal glucuronoyl esterases : Genome mining based enzyme discovery and biochemical characterization
Author: Dilokpimol, Adiphol; Makela, Miia R.; Cerullo, Gabriella; Zhou, Miaomiao; Varriale, Simona; Gidijala, Loknath; Bras, Joana L. A.; Jutten, Peter; Piechot, Alexander; Verhaert, Raymond; Faraco, Vincenza; Hilden, Kristiina S.; de Vries, Ronald P.
Contributor: University of Helsinki, Department of Microbiology
University of Helsinki, Fungal Genetics and Biotechnology
Date: 2018-01-25
Language: eng
Number of pages: 6
Belongs to series: New Biotechnology
ISSN: 1871-6784
URI: http://hdl.handle.net/10138/307189
Abstract: 4-O-Methyl-D-glucuronic acid (MeGlcA) is a side-residue of glucuronoarabinoxylan and can form ester linkages to lignin, contributing significantly to the strength and rigidity of the plant cell wall. Glucuronoyl esterases (4-O-methyl-glucuronoyl methylesterases, GEs) can cleave this ester bond, and therefore may play a significant role as auxiliary enzymes in biomass saccharification for the production of biofuels and biochemicals. GEs belong to a relatively new family of carbohydrate esterases (CE15) in the CAZy database (www.cazy.org), and so far around ten fungal GEs have been characterized. To explore additional GE enzymes, we used a genome mining strategy. BLAST analysis with characterized GEs against approximately 250 publicly accessible fungal genomes identified more than 150 putative fungal GEs, which were classified into eight phylogenetic sub-groups. To validate the genome mining strategy, 21 selected GEs from both ascomycete and basidiomycete fungi were heterologously produced in Pichia pastoris. Of these enzymes, 18 were active against benzyl D-glucuronate demonstrating the suitability of our genome mining strategy for enzyme discovery.
Subject: Glucuronoyl esterase
Glucuronic acid
Genome mining
Plant cell wall
Fungi
LIGNIN-CARBOHYDRATE COMPLEXES
MYCELIOPHTHORA-THERMOPHILA
ACID ESTER
MODEL
HEMICELLULOSES
416 Food Science
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