Fungal feruloyl esterases: Functional validation of genome mining based enzyme discovery including uncharacterized subfamilies

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http://hdl.handle.net/10138/307191

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Dilokpimol , A , Mäkelä , M R , Varriale , S , Zhou , M , Cerullo , G , Gidijala , L , Hinkka , H T , Brás , J L A , Jütten , P , Piechot , A , Verhaert , R , Hilden , S K , Faraco , V & de Vries , R 2018 , ' Fungal feruloyl esterases: Functional validation of genome mining based enzyme discovery including uncharacterized subfamilies ' , New Biotechnology , vol. 41 , pp. 9-14 . https://doi.org/10.1016/j.nbt.2017.11.004

Title: Fungal feruloyl esterases: Functional validation of genome mining based enzyme discovery including uncharacterized subfamilies
Author: Dilokpimol, Adiphol; Mäkelä, Miia Riitta; Varriale, Simona; Zhou, Miaomiao; Cerullo, Gabriella; Gidijala, Loknath; Hinkka, Harri Tapio; Brás, Joana L.A.; Jütten, Peter; Piechot, Alexander; Verhaert, Raymond; Hilden, Sari Kristiina; Faraco, Vincenza; de Vries, Ronald
Contributor organization: Department of Food and Nutrition
Fungal Genetics and Biotechnology
Department of Microbiology
Helsinki Institute of Sustainability Science (HELSUS)
Date: 2018-03-25
Language: eng
Number of pages: 6
Belongs to series: New Biotechnology
ISSN: 1871-6784
DOI: https://doi.org/10.1016/j.nbt.2017.11.004
URI: http://hdl.handle.net/10138/307191
Abstract: Feruloyl esterases (FAEs) are a diverse group of enzymes that specifically catalyze the hydrolysis of ester bonds between a hydroxycinnamic (e.g. ferulic) acid and plant poly- or oligosaccharides. FAEs as auxiliary enzymes significantly assist xylanolytic and pectinolytic enzymes in gaining access to their site of action during biomass saccharification for biofuel and biochemical production. A limited number of FAEs have been functionally characterized compared to over 1000 putative fungal FAEs that were recently predicted by similarity-based genome mining, which divided phylogenetically into different subfamilies (SFs). In this study, 27 putative and six characterized FAEs from both ascomycete and basidiomycete fungi were selected and heterologously expressed in Pichia pastoris and the recombinant proteins biochemically characterized to validate the previous genome mining and phylogenetical grouping and to expand the information on activity of fungal FAEs. As a result, 20 enzymes were shown to possess FAE activity, being active towards pNP-ferulate and/or methyl hydroxycinnamate substrates, and covering 11 subfamilies. Most of the new FAEs showed activities comparable to those of previously characterized fungal FAEs.
Subject: 1182 Biochemistry, cell and molecular biology
Feruloyl esterase
Ferulic acid
Genome mining
Plant cell wall
Fungi
CELL WALL POLYSACCHARIDES
ASPERGILLUS-NIGER
CRYSTAL-STRUCTURE
ACID
DEGRADATION
FAMILY
OLIGOSACCHARIDES
PURIFICATION
SPECIFICITY
PERFORMANCE
Feruloyl esterase
Ferulic acid
Genome mining
Plant cell wall
Fungi
CELL WALL POLYSACCHARIDES
ASPERGILLUS-NIGER
CRYSTAL-STRUCTURE
ACID
DEGRADATION
FAMILY
OLIGOSACCHARIDES
PURIFICATION
SPECIFICITY
PERFORMANCE
Peer reviewed: Yes
Rights: cc_by_nc_nd
Usage restriction: openAccess
Self-archived version: acceptedVersion


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