Induction of Genes Encoding Plant Cell Wall-Degrading Carbohydrate-Active Enzymes by Lignocellulose-Derived Monosaccharides and Cellobiose in the White-Rot Fungus Dichomitus squalens

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Lopez , S C , Peng , M , Issak , T Y , Daly , P , de Vries , R P & Mäkelä , M R 2018 , ' Induction of Genes Encoding Plant Cell Wall-Degrading Carbohydrate-Active Enzymes by Lignocellulose-Derived Monosaccharides and Cellobiose in the White-Rot Fungus Dichomitus squalens ' , Applied and Environmental Microbiology , vol. 84 , no. 11 , e00403-18 . https://doi.org/10.1128/AEM.00403-18

Title: Induction of Genes Encoding Plant Cell Wall-Degrading Carbohydrate-Active Enzymes by Lignocellulose-Derived Monosaccharides and Cellobiose in the White-Rot Fungus Dichomitus squalens
Author: Lopez, Sara Casado; Peng, Mao; Issak, Tedros Yonatan; Daly, Paul; de Vries, Ronald P.; Mäkelä, Miia R.
Contributor organization: Department of Microbiology
Date: 2018-06
Language: eng
Number of pages: 11
Belongs to series: Applied and Environmental Microbiology
ISSN: 0099-2240
DOI: https://doi.org/10.1128/AEM.00403-18
URI: http://hdl.handle.net/10138/307209
Abstract: Fungi can decompose plant biomass into small oligo-and monosaccharides to be used as carbon sources. Some of these small molecules may induce metabolic pathways and the production of extracellular enzymes targeted for degradation of plant cell wall polymers. Despite extensive studies in ascomycete fungi, little is known about the nature of inducers for the lignocellulolytic systems of basidiomycetes. In this study, we analyzed six sugars known to induce the expression of lignocellulolytic genes in ascomycetes for their role as inducers in the basidiomycete white-rot fungus Dichomitus squalens using a transcriptomic approach. This identified cellobiose and L-rhamnose as the main inducers of cellulolytic and pectinolytic genes, respectively, of D. squalens. Our results also identified differences in gene expression patterns between dikaryotic and monokaryotic strains of D. squalens cultivated on plant biomass-derived monosaccharides and the disaccharide cellobiose. This suggests that despite conservation of the induction between these two genetic forms of D. squalens, the fine-tuning in the gene regulation of lignocellulose conversion is differently organized in these strains. IMPORTANCE Wood-decomposing basidiomycete fungi have a major role in the global carbon cycle and are promising candidates for lignocellulosic biorefinery applications. However, information on which components trigger enzyme production is currently lacking, which is crucial for the efficient use of these fungi in biotechnology. In this study, transcriptomes of the white-rot fungus Dichomitus squalens from plant biomass-derived monosaccharide and cellobiose cultures were studied to identify compounds that induce the expression of genes involved in plant biomass degradation.
Subject: basidiomycetes
cellobiose
plant biomass degradation
regulation
rhamnose
ASPERGILLUS-NIGER
WOOD
BIOMASS
DECOMPOSITION
DEGRADATION
REVEALS
RELEASE
PECTIN
LIGNIN
1183 Plant biology, microbiology, virology
Peer reviewed: Yes
Usage restriction: openAccess
Self-archived version: acceptedVersion


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