Screening of glycoside hydrolases and ionic liquids for fibre modification

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Rahikainen , J , Anbarasan , S , Wahlström , R , Parviainen , A , King , A W T , Puranen , T , Kruus , K , Kilpeläinen , I , Turunen , O & Suurnäkki , A 2018 , ' Screening of glycoside hydrolases and ionic liquids for fibre modification ' , Journal of Chemical Technology and Biotechnology , vol. 93 , no. 3 , pp. 818–826 . https://doi.org/10.1002/jctb.5435

Title: Screening of glycoside hydrolases and ionic liquids for fibre modification
Author: Rahikainen, Jenni; Anbarasan, Sasikala; Wahlström, Ronny; Parviainen, Arno; King, Alistair W. T.; Puranen, Terhi; Kruus, Kristiina; Kilpeläinen, Ilkka; Turunen, Ossi; Suurnäkki, Anna
Contributor organization: Department of Chemistry
Synthesis and Analysis
Date: 2018-03
Language: eng
Number of pages: 9
Belongs to series: Journal of Chemical Technology and Biotechnology
ISSN: 0268-2575
DOI: https://doi.org/10.1002/jctb.5435
URI: http://hdl.handle.net/10138/307279
Abstract: BACKGROUNDThis study elaborates the possibility to apply combined ionic liquid (IL) and enzyme treatments for pulp fibre modification. The approach involves swelling of fibre surfaces with IL followed by enzymatic modification of the disrupted fibre surface using carbohydrate active enzymes. RESULTSThe capacity of seven cellulose-dissolving or cellulose-swelling ionic liquids to swell pulp fibres was compared. In addition, thirteen cellulases and five xylanases were screened for their IL tolerance, which determines their applicability in combined or sequential IL-enzyme treatments of fibres. Among the studied ionic liquids, 1-ethyl-3-methylimidazolium dimethylphosphate ([EMIM]DMP) and 1,3-dimethylimidazolium dimethylphosphate ([DMIM]DMP) had the strongest effect on fibre swelling. These solvents were also found to be the least inactivating for the studied enzymes. CONCLUSIONEnzyme compatibility and cellulose-dissolving capability are not two conflicting properties of an ionic liquid. (c) 2017 Society of Chemical Industry
Subject: 116 Chemical sciences
ionic liquid
cellulose
endoglucanase
xylanase
fibre modification
CBM
TRICHODERMA-REESEI CELLULASES
CELLULOSE HYDROLYSIS
ENZYMATIC-HYDROLYSIS
DISSOLVING PULP
GH10 XYLANASE
CORE DOMAINS
DISSOLUTION
ENDOGLUCANASES
GLYCOSYLATION
SPECIFICITY
Peer reviewed: Yes
Usage restriction: openAccess
Self-archived version: acceptedVersion


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