Immobilization of a phosphonium ionic liquid on a silica monolith forhydrophilic interaction chromatography

Show full item record



Permalink

http://hdl.handle.net/10138/307302

Citation

Moravcova , D , Planeta , J , King , A W T & Wiedmer , S K 2018 , ' Immobilization of a phosphonium ionic liquid on a silica monolith forhydrophilic interaction chromatography ' , Journal of Chromatography. A , vol. 1552 , pp. 53-59 . https://doi.org/10.1016/j.chroma.2018.04.012

Title: Immobilization of a phosphonium ionic liquid on a silica monolith forhydrophilic interaction chromatography
Author: Moravcova, Dana; Planeta, Josef; King, Alistair William Thomas; Wiedmer, Susanne Kristina
Contributor: University of Helsinki, Department of Chemistry
University of Helsinki, Department of Chemistry
Date: 2018-06-01
Language: eng
Number of pages: 7
Belongs to series: Journal of Chromatography. A
ISSN: 0021-9673
URI: http://hdl.handle.net/10138/307302
Abstract: A methodology for preparing phosphonium-based ionic liquid modified silica-based monolithic capillary columns is presented. The silica monolithic columns with dimensions of 150 × 0.1 mm were modified by a phosphonium-based ionic liquid (trioctyl(3/4-vinylbenzyl)phosphonium chloride) via3-(trimethoxysilyl)propyl methacrylate. The prepared columns were evaluated under hydrophilic inter-action liquid chromatography separation conditions, employing a sample mixture containing purine and pyrimidine bases and nucleosides. Detection was made by UV. The high efficiency of the original silica monolith was preserved even after modification, and it reached values in the range of 98,000–174,000 theoretical plates/m. The effects of the concentration of acetonitrile in the mobile phase, the presence of additives in the mobile phase, such as, acetic acid or ammonium acetate, and the pH of the mobile phaseon the separation of some selected analytes were investigated. The prepared columns showed different separation selectivity compared to silica, phenyl and sulfobetaine stationary phases.
Subject: 116 Chemical sciences
Silica monolithic capillary column
HILIC
Nucleobase
Nucleoside
TANDEM MASS-SPECTROMETRY
STATIONARY PHASES
URINARY NUCLEOSIDES
COLUMNS
SEPARATION
SELECTIVITY
CANCER
RNA
DNA
Rights:


Files in this item

Total number of downloads: Loading...

Files Size Format View
pre_print_Dana.pdf 1.837Mb PDF View/Open
Document_Immobilization2018.pdf 772.0Kb PDF View/Open

This item appears in the following Collection(s)

Show full item record