Moravcova , D , Planeta , J , King , A W T & Wiedmer , S K 2018 , ' Immobilization of a phosphonium ionic liquid on a silica monolith forhydrophilic interaction chromatography ' , Journal of Chromatography. A , vol. 1552 , pp. 53-59 . https://doi.org/10.1016/j.chroma.2018.04.012
Title: | Immobilization of a phosphonium ionic liquid on a silica monolith forhydrophilic interaction chromatography |
Author: | Moravcova, Dana; Planeta, Josef; King, Alistair William Thomas; Wiedmer, Susanne Kristina |
Contributor organization: | Department of Chemistry Susanne Wiedmer / Principal Investigator |
Date: | 2018-06-01 |
Language: | eng |
Number of pages: | 7 |
Belongs to series: | Journal of Chromatography. A |
ISSN: | 0021-9673 |
DOI: | https://doi.org/10.1016/j.chroma.2018.04.012 |
URI: | http://hdl.handle.net/10138/307302 |
Abstract: | A methodology for preparing phosphonium-based ionic liquid modified silica-based monolithic capillary columns is presented. The silica monolithic columns with dimensions of 150 × 0.1 mm were modified by a phosphonium-based ionic liquid (trioctyl(3/4-vinylbenzyl)phosphonium chloride) via3-(trimethoxysilyl)propyl methacrylate. The prepared columns were evaluated under hydrophilic inter-action liquid chromatography separation conditions, employing a sample mixture containing purine and pyrimidine bases and nucleosides. Detection was made by UV. The high efficiency of the original silica monolith was preserved even after modification, and it reached values in the range of 98,000–174,000 theoretical plates/m. The effects of the concentration of acetonitrile in the mobile phase, the presence of additives in the mobile phase, such as, acetic acid or ammonium acetate, and the pH of the mobile phaseon the separation of some selected analytes were investigated. The prepared columns showed different separation selectivity compared to silica, phenyl and sulfobetaine stationary phases. |
Subject: |
116 Chemical sciences
Silica monolithic capillary column HILIC Nucleobase Nucleoside TANDEM MASS-SPECTROMETRY STATIONARY PHASES URINARY NUCLEOSIDES COLUMNS SEPARATION SELECTIVITY CANCER RNA DNA |
Peer reviewed: | Yes |
Rights: | cc_by_nc_nd |
Usage restriction: | openAccess |
Self-archived version: | acceptedVersion |
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