Simultaneous Detection of Morphine and Codeine in the Presence of Ascorbic Acid and Uric Acid and in Human Plasma at Nafion Single-Walled Carbon Nanotube Thin-Film Electrode

Show full item record



Permalink

http://hdl.handle.net/10138/307425

Citation

Wester , N , Mynttinen , E , Etula , J , Lilius , T , Kalso , E , Kauppinen , E I , Laurila , T & Koskinen , J 2019 , ' Simultaneous Detection of Morphine and Codeine in the Presence of Ascorbic Acid and Uric Acid and in Human Plasma at Nafion Single-Walled Carbon Nanotube Thin-Film Electrode ' , ACS Omega , vol. 4 , no. 18 , pp. 17726-17734 . https://doi.org/10.1021/acsomega.9b02147

Title: Simultaneous Detection of Morphine and Codeine in the Presence of Ascorbic Acid and Uric Acid and in Human Plasma at Nafion Single-Walled Carbon Nanotube Thin-Film Electrode
Author: Wester, Niklas; Mynttinen, Elsi; Etula, Jarkko; Lilius, Tuomas; Kalso, Eija; Kauppinen, Esko I.; Laurila, Tomi; Koskinen, Jari
Contributor: University of Helsinki, HUSLAB
University of Helsinki, HUS Perioperative, Intensive Care and Pain Medicine
Date: 2019-10-29
Language: eng
Number of pages: 9
Belongs to series: ACS Omega
ISSN: 2470-1343
URI: http://hdl.handle.net/10138/307425
Abstract: In clinical settings, the dosing and differential diagnosis of the poisoning of morphine (MO) and codeine (CO) is challenging due to interindividual variations in metabolism. However, direct electrochemical detection of these analytes from biological matrices is inherently challenging due to interference from large concentrations of anions, such as ascorbic acid (AA) and uric acid (UA), as well as fouling of the electrode by proteins. In this work, a disposable Nafion-coated single-walled carbon nanotube network (SWCNT) electrode was developed. We show facile electron transfer and efficient charge separation between the interfering anions and positively charged MO and CO, as well as significantly reduced matrix effect in human plasma. The Nafion coating alters the voltammetric response of MO and CO, enabling simultaneous detection. With this SWCNT/Nafion electrode, two linear ranges of 0.05-1 and 1-10 mu M were found for MO and one linear range of 0.1-50 mu M for CO. Moreover, the selective and simultaneous detection of MO and CO was achieved in large excess of AA and UA, as well as, for the first time, in unprocessed human plasma. The favorable properties of this electrode enabled measurements in plasma with only mild dilution and without the precipitation of proteins.
Subject: ELECTROCHEMICAL DETERMINATION
VOLTAMMETRIC OXIDATION
RAMAN-SPECTROSCOPY
PHARMACOKINETICS
METABOLITE
DRUGS
ACETAMINOPHEN
MEMBRANES
DOPAMINE
BEHAVIOR
3111 Biomedicine
116 Chemical sciences
Rights:


Files in this item

Total number of downloads: Loading...

Files Size Format View
acsomega.9b02147.pdf 2.502Mb PDF View/Open

This item appears in the following Collection(s)

Show full item record