Cultivation of Podospora anserina on soybean hulls results in an efficient enzyme cocktail for plant biomass hydrolysis

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Mäkelä , M R , Bouzid , O , Robl , D , Post , H , Peng , M , Heck , A , Altelaar , M & de Vries , R P 2017 , ' Cultivation of Podospora anserina on soybean hulls results in an efficient enzyme cocktail for plant biomass hydrolysis ' , New Biotechnology , vol. 37 , pp. 162-171 . https://doi.org/10.1016/j.nbt.2017.02.002

Title: Cultivation of Podospora anserina on soybean hulls results in an efficient enzyme cocktail for plant biomass hydrolysis
Author: Mäkelä, Miia R.; Bouzid, Ourdia; Robl, Diogo; Post, Harm; Peng, Mao; Heck, Albert; Altelaar, Maarten; de Vries, Ronald P.
Contributor organization: Department of Food and Nutrition
University of Helsinki
Date: 2017-07-25
Language: eng
Number of pages: 10
Belongs to series: New Biotechnology
ISSN: 1871-6784
DOI: https://doi.org/10.1016/j.nbt.2017.02.002
URI: http://hdl.handle.net/10138/307437
Abstract: The coprophilic ascomycete fungus Podospora anserina was cultivated on three different plant biomasses, i.e. cotton seed hulls (CSH), soybean hulls (SBH) and acid-pretreated wheat straw (WS) for four days, and the potential of the produced enzyme mixtures was compared in the enzymatic saccharification of the corresponding lignocellulose feedstocks. The enzyme cocktail P. anserina produced after three days of growth on SBH showed superior capacity to release reducing sugars from all tested plant biomass feedstocks compared to the enzyme mixtures from CSH and WS cultures. Detailed proteomics analysis of the culture supernatants revealed that SBH contained the most diverse set of enzymes targeted on plant cell wall polymers and was particularly abundant in xylan, mannan and pectin acting enzymes. The importance of lytic polysaccharide monooxygenases (LPMOs) in plant biomass deconstruction was supported by identification of 20 out of 33 AA9 LPMOs in the SBH cultures. The results highlight the suitability of P. anserina as a source of plant cell wall degrading enzymes for biotechnological applications and the importance of selecting the most optimal substrate for the production of enzyme mixtures. (C) 2017 Elsevier B.V. All rights reserved.
Subject: Proteomics
Saccharification
Plant biomass
Podospora anserina
Carbohydrate active enzymes
Auxiliary activities
LYTIC POLYSACCHARIDE MONOOXYGENASES
CELLOBIOSE DEHYDROGENASES
OXIDATIVE-DEGRADATION
TRICHODERMA-REESEI
ASPERGILLUS-NIGER
ACTIVE ENZYMES
SACCHARIFICATION
CELLULOSE
FAMILY
FUNGI
1182 Biochemistry, cell and molecular biology
1183 Plant biology, microbiology, virology
Peer reviewed: Yes
Rights: cc_by_nc_nd
Usage restriction: openAccess
Self-archived version: acceptedVersion


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