Correlation between Ionic Liquid Cytotoxicity and Liposome-Ionic Liquid Interactions

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http://hdl.handle.net/10138/307532

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Ruokonen , S-K , Sanwald , C , Robciuc , A , Hietala , S , Rantamäki , A H , Witos , J , King , A W T , Lämmerhofer , M & Wiedmer , S K 2018 , ' Correlation between Ionic Liquid Cytotoxicity and Liposome-Ionic Liquid Interactions ' , Chemistry: A European Journal , vol. 24 , no. 11 , pp. 2669-2680 . https://doi.org/10.1002/chem.201704924

Title: Correlation between Ionic Liquid Cytotoxicity and Liposome-Ionic Liquid Interactions
Author: Ruokonen, Suvi-Katriina; Sanwald, Corinna; Robciuc, Alexandra; Hietala, Sami; Rantamäki, Antti H.; Witos, Joanna; King, Alistair W. T.; Lämmerhofer, Michael; Wiedmer, Susanne K.
Contributor: University of Helsinki, Department of Chemistry
University of Helsinki, Silmäklinikka
University of Helsinki, Department of Chemistry
University of Helsinki, Department of Chemistry
University of Helsinki, Department of Chemistry
University of Helsinki, Department of Chemistry
University of Helsinki, Department of Chemistry
Date: 2018-02-21
Language: eng
Number of pages: 12
Belongs to series: Chemistry: A European Journal
ISSN: 0947-6539
URI: http://hdl.handle.net/10138/307532
Abstract: This study aims at extending the understanding of the toxicity mechanism of ionic liquids (ILs) using various analytical methods and cytotoxicity assays. The cytotoxicity of eight ILs and one zwitterionic compound was determined using mammalian and bacterial cells. The time dependency of the IL toxicity was assessed using human corneal epithelial cells. Hemolysis was performed using human red blood cells and the results were compared with destabilization data of synthetic liposomes upon addition of ILs. The effect of the ILs on the size and zeta potential of liposomes revealed information on changes in the lipid bilayer. Differential scanning calorimetry was used to study the penetration of the ILs into the lipid bilayer. Pulsed field gradient nuclear magnetic resonance spectroscopy was used to determine whether the ILs occurred as unimers, micelles, or if they were bound to liposomes. The results show that the investigated ILs can be divided into three groups based on the cytotoxicity mechanism: cell wall disrupting ILs, ILs exerting toxicity through both cell wall penetration and metabolic alteration, and ILs affecting solely on cell metabolism.
Subject: analytical methods
cytotoxicity
ionic liquids
liposomes
toxicology
IN-VITRO
TOXICITY
AGGREGATION
IMPACT
CELLS
BIOLUMINESCENCE
SOLUBILIZATION
CALORIMETRY
SCATTERING
MEMBRANES
116 Chemical sciences
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