Give me a sample of air and I will tell which species are found from your region : Molecular identification of fungi from airborne spore samples

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Abrego , N , Norros , V , Halme , P , Somervuo , P , Ali-Kovero , H & Ovaskainen , O 2018 , ' Give me a sample of air and I will tell which species are found from your region : Molecular identification of fungi from airborne spore samples ' , Molecular Ecology Resources , vol. 18 , no. 3 , pp. 511-524 . https://doi.org/10.1111/1755-0998.12755

Title: Give me a sample of air and I will tell which species are found from your region : Molecular identification of fungi from airborne spore samples
Author: Abrego, Nerea; Norros, Veera; Halme, Panu; Somervuo, Panu; Ali-Kovero, Heini; Ovaskainen, Otso
Contributor organization: Department of Agricultural Sciences
Spatial Foodweb Ecology Group
Research Centre for Ecological Change
Biosciences
Organismal and Evolutionary Biology Research Programme
Ecology and Evolutionary Biology
Otso Ovaskainen / Principal Investigator
Plant Production Sciences
Date: 2018-05
Language: eng
Number of pages: 14
Belongs to series: Molecular Ecology Resources
ISSN: 1755-098X
DOI: https://doi.org/10.1111/1755-0998.12755
URI: http://hdl.handle.net/10138/307606
Abstract: Fungi are a megadiverse group of organisms, they play major roles in ecosystem functioning and are important for human health, food production and nature conservation. Our knowledge on fungal diversity and fungal ecology is however still very limited, in part because surveying and identifying fungi is time demanding and requires expert knowledge. We present a method that allows anyone to generate a list of fungal species likely to occur in a region of interest, with minimal effort and without requiring taxonomical expertise. The method consists of using a cyclone sampler to acquire fungal spores directly from the air to an Eppendorf tube, and applying DNA barcoding with probabilistic species identification to generate a list of species from the sample. We tested the feasibility of the method by acquiring replicate air samples from different geographical regions within Finland. Our results show that air sampling is adequate for regional-level surveys, with samples collected >100km apart varying but samples collected
Subject: atmospheric diversity
fungal diversity
fungal sampling method
fungal survey
molecular identification
spore
WOOD-INHABITING FUNGI
COMMUNITY COMPOSITION
TEMPORAL VARIABILITY
DISPERSAL LIMITATION
LANDSCAPE SCALE
SHORT DISTANCES
OUTDOOR AIR
ATMOSPHERE
DIVERSITY
BACTERIAL
1182 Biochemistry, cell and molecular biology
1181 Ecology, evolutionary biology
Peer reviewed: Yes
Usage restriction: openAccess
Self-archived version: publishedVersion


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