TILLING, high-resolution melting (HRM), and next-generation sequencing (NGS) techniques in plant mutation breeding

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Taheri , S , Abdullah , T L , Jain , S M , Sahebi , M & Azizi , P 2017 , ' TILLING, high-resolution melting (HRM), and next-generation sequencing (NGS) techniques in plant mutation breeding ' , Molecular Breeding , vol. 37 , no. 3 , 40 . https://doi.org/10.1007/s11032-017-0643-7

Title: TILLING, high-resolution melting (HRM), and next-generation sequencing (NGS) techniques in plant mutation breeding
Author: Taheri, Sima; Abdullah, Thohirah Lee; Jain, Shri Mohan; Sahebi, Mahbod; Azizi, Parisa
Contributor organization: Department of Agricultural Sciences
Date: 2017-03
Language: eng
Number of pages: 23
Belongs to series: Molecular Breeding
ISSN: 1380-3743
DOI: https://doi.org/10.1007/s11032-017-0643-7
URI: http://hdl.handle.net/10138/307693
Abstract: Induced mutations have been used effectively for plant improvement. Physical and chemical mutagens induce a high frequency of genome variation. Recently, developed screening methods have allowed the detection of single nucleotide polymorphisms (SNPs) and the identification of traits that are difficult to identify at the molecular level by conventional breeding. With the assistance of reverse genetic techniques, sequence variation information can be linked to traits to investigate gene function. Targeting induced local lesions in genomes (TILLING) is a high-throughput technique to identify single nucleotide mutations in a specific region of a gene of interest with a powerful detection method resulted from chemical-induced mutagenesis. The main advantage of TILLING as a reverse genetics strategy is that it can be applied to any species, regardless of genome size and ploidy level. However, TILLING requires laborious and time-consuming steps, and a lack of complete genome sequence information for many crop species has slowed the development of suitable TILLING targets. Another method, high-resolution melting (HRM), which has assisted TILLING in mutation detection, is faster, simpler and less expensive with non-enzymatic screening system. Currently, the sequencing of crop genomes has completely changed our vision and interpretation of genome organization and evolution. Impressive progress in next-generation sequencing (NGS) technologies has paved the way for the detection and exploitation of genetic variation in a given DNA or RNA molecule. This review discusses the applications of TILLING in combination with HRM and NGS technologies for screening of induced mutations and discovering SNPs in mutation breeding programs.
Subject: Mutation breeding
Mutagenesis
EMS
TILLING
High resolution melting
Next-generation sequencing
SNPs
EMS-INDUCED MUTATIONS
PERFORMANCE LIQUID-CHROMATOGRAPHY
NUCLEOTIDE POLYMORPHISM DISCOVERY
CHEMICALLY-INDUCED MUTATIONS
MARKER-ASSISTED SELECTION
BROAD-SPECTRUM RESISTANCE
DETECT INDUCED MUTATIONS
INDUCED POINT MUTATIONS
REVERSE GENETICS TOOL
ARABIDOPSIS-THALIANA
414 Agricultural biotechnology
Peer reviewed: Yes
Usage restriction: openAccess
Self-archived version: publishedVersion


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