Improved characterization of Clematis based on new chloroplast microsatellite markers and nuclear ITS sequences

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Liu , Z & Korpelainen , H 2018 , ' Improved characterization of Clematis based on new chloroplast microsatellite markers and nuclear ITS sequences ' , Horticulture environment and biotechnology , vol. 59 , no. 6 , pp. 889-897 . https://doi.org/10.1007/s13580-018-0090-3

Title: Improved characterization of Clematis based on new chloroplast microsatellite markers and nuclear ITS sequences
Author: Liu, Zhigao; Korpelainen, Helena
Contributor: University of Helsinki, Viikki Plant Science Centre (ViPS)
Date: 2018-12
Language: eng
Number of pages: 9
Belongs to series: Horticulture environment and biotechnology
ISSN: 2211-3452
URI: http://hdl.handle.net/10138/307841
Abstract: Currently, there is a lack of genetic markers capable of effectively detecting polymorphisms in Clematis. Therefore, we developed new markers to investigate inter- and intraspecific diversity in Clematis. Based on the complete chloroplast genome of Clematis terniflora, simple sequence repeats were explored and primer pairs were designed for all ten adequate repeat regions (cpSSRs), which were tested in 43 individuals of 11 Clematis species. In addition, the nuclear ITS region was sequenced in 11 Clematis species. Seven cpSSR loci were found to be polymorphic in the genus and serve as markers that can distinguish different species and be used in different genetic analyses, including cultivar identification to assist the breeding of new ornamental cultivars.
Subject: Genetic markers
Genetic variation
Molecular identification
Ornamental plants
genetic relationships
DNA
RANUNCULACEAE
PHYLOGENY
GENOMES
CLASSIFICATION
FREQUENCY
REPEATS
RATES
1181 Ecology, evolutionary biology
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