Quantification of roots in soil, plant-fungal symbioses and in mixed plant populations with real-time PCR

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dc.contributor.author Kalendar, Ruslan
dc.contributor.author Vottonen, Laura Linnea
dc.contributor.author Seppänen, Mervi Mirjam
dc.date.accessioned 2019-12-04T10:54:02Z
dc.date.available 2019-12-04T10:54:02Z
dc.date.issued 2019-06-27
dc.identifier.citation Kalendar , R , Vottonen , L L & Seppänen , M M 2019 , ' Quantification of roots in soil, plant-fungal symbioses and in mixed plant populations with real-time PCR ' , Paper presented at EGF-EUCARPIA Joint Symposium 2019 , Zurich , Switzerland , 24/06/2019 - 27/06/2019 pp. 458-460 . https://doi.org/10.3929/ethz-b-000369264
dc.identifier.citation conference
dc.identifier.other PURE: 123466565
dc.identifier.other PURE UUID: 8d5b9263-c71c-4984-a28e-4ff6b06a37ad
dc.identifier.other ORCID: /0000-0003-3986-2460/work/108463595
dc.identifier.uri http://hdl.handle.net/10138/307883
dc.description.abstract Plant-soil and underground plant-plant interactions have direct implications for the conservation of biodiversity, plant productivity and understanding of local ecosystem responses to global environmental change. Study of associated organisms in the soil, symbiosis and in mixed plant populations, is limited by difficulties in the species quantification and sensitive detection. We have designed and tested new PCR primers and hydrolysis probes for TaqMan assays that target the nuclear ribosomal gene plant DNA. Species-specific probes and universal primers were developed for the monocots and dicots plant species and evaluated for specificity and sensitivity in TaqMan assays on DNA extracted from soil and in mixed plant populations. The assays were sensitive and able to quantify rear targets in mixed plant populations. This approach appears to offer a quick and sensitive way to study roots in soil, symbiosis and in mixed plant populations composition and could provide insights into root function and responses not readily provided by other methods. These features identify the new primer sets developed in this study as useful complements to other universal primers for the study of plant diversity and community composition. en
dc.format.extent 3
dc.language.iso eng
dc.rights cc_by_nc
dc.rights cc_by_nd
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject 4111 Agronomy
dc.title Quantification of roots in soil, plant-fungal symbioses and in mixed plant populations with real-time PCR en
dc.type Paper
dc.contributor.organization Crop Science Research Group
dc.contributor.organization Department of Agricultural Sciences
dc.contributor.organization Helsinki Institute of Sustainability Science (HELSUS)
dc.contributor.organization Viikki Plant Science Centre (ViPS)
dc.contributor.organization University Management
dc.contributor.organization Forages for the future
dc.contributor.organization Plant Production Sciences
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.3929/ethz-b-000369264
dc.rights.accesslevel openAccess
dc.type.version publishedVersion
dc.type.version publishedVersion
dc.identifier.url https://www.egfeucarpia2019.ch/
dc.identifier.url https://www.europeangrassland.org/en/infos/printed-matter/proceedings.html
dc.identifier.url https://www.research-collection.ethz.ch/handle/20.500.11850/369264
dc.identifier.url https://www.europeangrassland.org/fileadmin/documents/Infos/Printed_Matter/Proceedings/EGF2019.pdf

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