Horizontal Gene Transfer of Phytochelatin Synthases from Bacteria to Extremophilic Green Algae

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Olsson , S , Penacho , V , Puente-Sanchez , F , Diaz , S , Eduardo Gonzalez-Pastor , J & Aguilera , A 2017 , ' Horizontal Gene Transfer of Phytochelatin Synthases from Bacteria to Extremophilic Green Algae ' , Microbial Ecology , vol. 73 , no. 1 , pp. 50-60 . https://doi.org/10.1007/s00248-016-0848-z

Title: Horizontal Gene Transfer of Phytochelatin Synthases from Bacteria to Extremophilic Green Algae
Author: Olsson, Sanna; Penacho, Vanessa; Puente-Sanchez, Fernando; Diaz, Silvia; Eduardo Gonzalez-Pastor, Jose; Aguilera, Angeles
Contributor: University of Helsinki, Department of Agricultural Sciences
Date: 2017-01
Language: eng
Number of pages: 11
Belongs to series: Microbial Ecology
ISSN: 0095-3628
URI: http://hdl.handle.net/10138/307903
Abstract: Transcriptomic sequencing together with bioinformatic analyses and an automated annotation process led us to identify novel phytochelatin synthase (PCS) genes from two extremophilic green algae (Chlamydomonas acidophila and Dunaliella acidophila). These genes are of intermediate length compared to known PCS genes from eukaryotes and PCS-like genes from prokaryotes. A detailed phylogenetic analysis gives new insight into the complicated evolutionary history of PCS genes and provides evidence for multiple horizontal gene transfer events from bacteria to eukaryotes within the gene family. A separate subgroup containing PCS-like genes within the PCS gene family is not supported since the PCS genes are monophyletic only when the PCS-like genes are included. The presence and functionality of the novel genes in the organisms were verified by genomic sequencing and qRT-PCR. Furthermore, the novel PCS gene in Chlamydomonas acidophila showed very strong induction by cadmium. Cloning and expression of the gene in Escherichia coli clearly improves its cadmium resistance. The gene in Dunaliella was not induced, most likely due to gene duplication.
Subject: Heavy metal tolerance
Cadmium
Acidophile
Chlamydomonas acidophila
Dunaliella acidophila
CADMIUM
TOLERANCE
EVOLUTION
PLANTS
METALLOTHIONEINS
CONSEQUENCES
ENVIRONMENT
MECHANISMS
MICROALGAE
INFERENCE
1183 Plant biology, microbiology, virology
1181 Ecology, evolutionary biology
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