Diversity of indoor fungi as revealed by DNA metabarcoding

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Korpelainen , H & Pietiläinen , M 2017 , ' Diversity of indoor fungi as revealed by DNA metabarcoding ' , Genome , vol. 60 , no. 1 , pp. 55-64 . https://doi.org/10.1139/gen-2015-0191

Title: Diversity of indoor fungi as revealed by DNA metabarcoding
Author: Korpelainen, Helena; Pietiläinen, Maria
Contributor: University of Helsinki, Department of Agricultural Sciences
University of Helsinki, Department of Agricultural Sciences
Date: 2017-01
Language: eng
Number of pages: 10
Belongs to series: Genome
ISSN: 0831-2796
URI: http://hdl.handle.net/10138/307915
Abstract: In the present study, we conducted DNA metabarcoding (the nuclear ITS2 region) for indoor fungal samples originating from two nursery schools with a suspected mould problem (sampling before and after renovation), from two university buildings, and from an old farmhouse. Good-quality sequences were obtained, and the results showed that DNA metabarcoding provides high resolution in fungal identification. The pooled proportions of sequences representing filamentous ascomycetes, filamentous basidiomycetes, yeasts, and other fungi equalled 62.3%, 8.0%, 28.3%, and 1.4%, respectively, and the total number of fungal genera found during the study was 585. When comparing fungal diversities and taxonomic composition between different types of buildings, no obvious pattern was detected. The average pairwise values of Sorensen(Chao) indices that were used to compare similarities for taxon composition between samples among the samples from the two university buildings, two nurseries, and farmhouse equaled 0.693, 0.736, 0.852, 0.928, and 0.981, respectively, while the mean similarity index for all samples was 0.864. We discovered that making explicit conclusions on the relationship between the indoor air quality and mycoflora is complicated by the lack of appropriate indicators for air quality and by the occurrence of wide spatial and temporal changes in diversity and compositions among samples.
Subject: indoor air quality
fungi
metabarcoding
next generation sequencing
RESPIRATORY HEALTH
ENVIRONMENTAL DNA
CHILDHOOD ASTHMA
BIODIVERSITY
HOMES
SCHOOLCHILDREN
COMMUNITIES
BUILDINGS
CHILDREN
DAMPNESS
1184 Genetics, developmental biology, physiology
1183 Plant biology, microbiology, virology
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