Biodiversity of pollen in indoor air samples as revealed by DNA metabarcoding

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dc.contributor.author Korpelainen, Helena
dc.contributor.author Pietiläinen, Maria
dc.date.accessioned 2019-12-04T15:22:01Z
dc.date.available 2019-12-04T15:22:01Z
dc.date.issued 2017-10
dc.identifier.citation Korpelainen , H & Pietiläinen , M 2017 , ' Biodiversity of pollen in indoor air samples as revealed by DNA metabarcoding ' , Nordic Journal of Botany , vol. 35 , no. 5 , pp. 602-608 . https://doi.org/10.1111/njb.01623
dc.identifier.other PURE: 92788031
dc.identifier.other PURE UUID: cfde43fe-678d-4140-85c9-d103b0c6d56b
dc.identifier.other WOS: 000413169200013
dc.identifier.other Scopus: 85031489831
dc.identifier.other ORCID: /0000-0002-7046-6995/work/66032997
dc.identifier.uri http://hdl.handle.net/10138/307916
dc.description.abstract We conducted DNA metabarcoding (based on the nuclear ITS2 region) to characterize indoor pollen samples (possibly accompanied by other plant fragments) and to discover whether there are seasonal changes in their taxonomic diversity. It was shown that DNA metabarcoding has potential to allow a good discovery of taxonomic diversity. The number of spermatophyte families and genera varied greatly among sampling sites (pooled results per building) and times, between 9-40 and 10-66, respectively. Comparable Shannon's diversity indices equaled 0.33-2.76 and 0.94-3.16. The total number of spermatophyte genera found during the study was 187, of which 43.9, 39.6, 7.5 and 9.1% represented wild, garden/crop and indoor house plants, and non-domestic fruit or other plant material, respectively. Comparable proportions of individual sequences equaled 77.4, 18.8, 2.7 and 1.1%, respectively. When comparing plant diversities and taxonomic composition among buildings or between seasons, no obvious pattern was detected, except for the second summer, when pollen coming from outdoors was highly dominant and the proportions of likely allergens, birch, grass, alder and mugwort pollen, were very high. The average pairwise values of SOrensen(Chao) indices that were used to compare similarities for taxon composition between samples among the samples from the two university buildings, two nurseries and farmhouse equaled 0.514, 0.109, 0.564, 0.865 and 0.867, respectively, while the mean similarity index for all samples was 0.524. Cleaning frequency may strongly contribute to the observed diversity. The discovery of considerable diversities, including pollen coming from outside, in both winter and summer shows that substantial amounts of pollen produced in summer enter buildings and stay there throughout the year. en
dc.format.extent 7
dc.language.iso eng
dc.relation.ispartof Nordic Journal of Botany
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject FUNGI
dc.subject PLATFORM
dc.subject BIOLOGY
dc.subject 1183 Plant biology, microbiology, virology
dc.title Biodiversity of pollen in indoor air samples as revealed by DNA metabarcoding en
dc.type Article
dc.contributor.organization Department of Agricultural Sciences
dc.contributor.organization Population Genetics and Biodiversity Group
dc.contributor.organization Viikki Plant Science Centre (ViPS)
dc.contributor.organization Plant Production Sciences
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.1111/njb.01623
dc.relation.issn 0107-055X
dc.rights.accesslevel openAccess
dc.type.version acceptedVersion

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