Expanding the feruloyl esterase gene family of Aspergillus niger by characterization of a feruloyl esterase, FaeC

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Dilokpimol , A , Mäkelä , M R , Mansouri , S , Belova , O , Waterstraat , M , Bunzel , M , de Vries , R P & Hilden , K S 2017 , ' Expanding the feruloyl esterase gene family of Aspergillus niger by characterization of a feruloyl esterase, FaeC ' , New Biotechnology , vol. 37 , pp. 200-209 . https://doi.org/10.1016/j.nbt.2017.02.007

Title: Expanding the feruloyl esterase gene family of Aspergillus niger by characterization of a feruloyl esterase, FaeC
Author: Dilokpimol, Adiphol; Mäkelä, Miia R.; Mansouri, Sadegh; Belova, Olga; Waterstraat, Martin; Bunzel, Mirko; de Vries, Ronald P.; Hilden, Kristiina S.
Contributor: University of Helsinki, Department of Food and Nutrition
University of Helsinki, Fungal Genetics and Biotechnology
University of Helsinki, Department of Food and Nutrition
University of Helsinki, Department of Food and Nutrition
Date: 2017-07-25
Language: eng
Number of pages: 10
Belongs to series: New Biotechnology
ISSN: 1871-6784
URI: http://hdl.handle.net/10138/308036
Abstract: A feruloyl esterase (FAE) from Aspergillus niger N402, FaeC was heterologously produced in Pichia pastoris X-33 in a yield of 10 mg/L. FaeC was most active at pH 7.0 and 50 degrees C, and showed broad substrate specificity and catalyzed the hydrolysis of methyl 3,4-dimethoxycinnamate, ethyl ferulate, methyl ferulate, methyl p-coumarate, ethyl coumarate, methyl sinapate, and methyl caffeate. The enzyme released both ferulic acid and p-coumaric acid from wheat arabinoxylan and sugar beet pectin (up to 3 mg/g polysaccharide), and acted synergistically with a commercial xylanase increasing the release of ferulic acid up to six-fold. The expression of faeC increased over time in the presence of feruloylated polysaccharides. Cinnamic, syringic, caffeic, vanillic and ferulic acid induced the expression of faeC. Overall expression of faeC was very low in all tested conditions, compared to two other A. niger FAE encoding genes, faeA and faeB. Our data showed that the fae genes responded differently towards the feruloylated polysaccharides and tested monomeric phenolic compounds suggesting that the corresponding FAE isoenzymes may target different substrates in a complementary manner. This may increase the efficiency of the degradation of diverse plant biomass. (C) 2017 Elsevier B.V. All rights reserved.
Subject: Aspergillus niger
Ferulic acid
Feruloyl esterase
Pectin
Xylan
SUGAR-BEET PULP
CELL-WALL POLYSACCHARIDES
ACID ESTERASES
DEGRADATION
ENZYME
CLASSIFICATION
PURIFICATION
BIOMASS
OLIGOSACCHARIDES
EXPRESSION
1182 Biochemistry, cell and molecular biology
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