Evaluation of potential genetic and chemical markers for Scots pine tolerance against Heterobasidion annosum infection

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http://hdl.handle.net/10138/308055

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Mukrimin , M , Kovalchuk , A , Ghimire , R P , Kivimaenpaa , M , Sun , H , Holopainen , J K & Asiegbu , F O 2019 , ' Evaluation of potential genetic and chemical markers for Scots pine tolerance against Heterobasidion annosum infection ' , Planta , vol. 250 , no. 6 , pp. 1881-1895 . https://doi.org/10.1007/s00425-019-03270-8

Julkaisun nimi: Evaluation of potential genetic and chemical markers for Scots pine tolerance against Heterobasidion annosum infection
Tekijä: Mukrimin, Mukrimin; Kovalchuk, Andriy; Ghimire, Rajendra P.; Kivimaenpaa, Minna; Sun, Hui; Holopainen, Jarmo K.; Asiegbu, Fred O.
Tekijän organisaatio: Department of Forest Sciences
Forest Ecology and Management
Helsinki Institute of Sustainability Science (HELSUS)
Viikki Plant Science Centre (ViPS)
Frederick Asiegbu / Principal Investigator
Päiväys: 2019-12
Kieli: eng
Sivumäärä: 15
Kuuluu julkaisusarjaan: Planta
ISSN: 0032-0935
DOI-tunniste: https://doi.org/10.1007/s00425-019-03270-8
URI: http://hdl.handle.net/10138/308055
Tiivistelmä: Main conclusion Two terpene compounds and four genes were identified as potential biomarkers for further evaluation for Scots pine susceptibility or tolerance against Heterobasidion annosum. Scots pine (Pinus sylvestris) is one of the main sources of timber in the boreal zone of Eurasia. Commercial pine plantations are vulnerable to root and butt rot disease caused by the fungus Heterobasidion annosum. The pathogen affects host growth rate, causes higher mortality and decreases in timber quality, resulting in considerable economic losses to forest owners. Genetic and biochemical factors contributing to Scots pine tolerance against H. annosum infection are not well understood. We assessed the predictive values of a set of potential genetic and chemical markers in a field experiment. We determined the expression levels of 25 genes and the concentrations of 36 terpenoid compounds in needles of 16 Scots pine trees randomly selected from a natural population prior to artificial infection. Stems of the same trees were artificially inoculated with H. annosum, and the length of necrotic lesions was documented 5 months post inoculation. Higher expression level of four genes included in our analysis and encoding predicted alpha-pinene synthase (two genes), geranyl diphosphate synthase (GPPS), and metacaspase 5 (MC5), could be associated with trees exhibiting increased levels of necrotic lesion formation in response to fungal inoculation. In contrast, concentrations of two terpenoid compounds, beta-caryophyllene and alpha-humulene, showed significant negative correlations with the lesion size. Further studies with larger sample size will help to elucidate new biomarkers or clarify the potential of the evaluated markers for use in Scots pine disease resistance breeding programs.
Avainsanat: Chemical defences
Gene expression
Heterobasidion
Root rot disease
Scots pine
Terpenoid
PICEA-ABIES CLONES
SESQUITERPENE SYNTHASES
WOOD TERPENOIDS
DROUGHT STRESS
PARVIPORUM
CONIFER
SUSCEPTIBILITY
EXPRESSION
DEFENSES
MONOTERPENES
1183 Plant biology, microbiology, virology
1182 Biochemistry, cell and molecular biology
Vertaisarvioitu: Kyllä
Tekijänoikeustiedot: cc_by
Pääsyrajoitteet: openAccess
Rinnakkaistallennettu versio: publishedVersion


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