Evaluation of potential genetic and chemical markers for Scots pine tolerance against Heterobasidion annosum infection

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dc.contributor.author Mukrimin, Mukrimin
dc.contributor.author Kovalchuk, Andriy
dc.contributor.author Ghimire, Rajendra P.
dc.contributor.author Kivimaenpaa, Minna
dc.contributor.author Sun, Hui
dc.contributor.author Holopainen, Jarmo K.
dc.contributor.author Asiegbu, Fred O.
dc.date.accessioned 2019-12-11T08:05:04Z
dc.date.available 2019-12-11T08:05:04Z
dc.date.issued 2019-12
dc.identifier.citation Mukrimin , M , Kovalchuk , A , Ghimire , R P , Kivimaenpaa , M , Sun , H , Holopainen , J K & Asiegbu , F O 2019 , ' Evaluation of potential genetic and chemical markers for Scots pine tolerance against Heterobasidion annosum infection ' , Planta , vol. 250 , no. 6 , pp. 1881-1895 . https://doi.org/10.1007/s00425-019-03270-8
dc.identifier.other PURE: 128845683
dc.identifier.other PURE UUID: 1a33aed1-9066-471b-94e0-8b07c7955680
dc.identifier.other WOS: 000496035900007
dc.identifier.other ORCID: /0000-0003-0223-7194/work/66032863
dc.identifier.other ORCID: /0000-0001-8704-4644/work/66034561
dc.identifier.uri http://hdl.handle.net/10138/308055
dc.description.abstract Main conclusion Two terpene compounds and four genes were identified as potential biomarkers for further evaluation for Scots pine susceptibility or tolerance against Heterobasidion annosum. Scots pine (Pinus sylvestris) is one of the main sources of timber in the boreal zone of Eurasia. Commercial pine plantations are vulnerable to root and butt rot disease caused by the fungus Heterobasidion annosum. The pathogen affects host growth rate, causes higher mortality and decreases in timber quality, resulting in considerable economic losses to forest owners. Genetic and biochemical factors contributing to Scots pine tolerance against H. annosum infection are not well understood. We assessed the predictive values of a set of potential genetic and chemical markers in a field experiment. We determined the expression levels of 25 genes and the concentrations of 36 terpenoid compounds in needles of 16 Scots pine trees randomly selected from a natural population prior to artificial infection. Stems of the same trees were artificially inoculated with H. annosum, and the length of necrotic lesions was documented 5 months post inoculation. Higher expression level of four genes included in our analysis and encoding predicted alpha-pinene synthase (two genes), geranyl diphosphate synthase (GPPS), and metacaspase 5 (MC5), could be associated with trees exhibiting increased levels of necrotic lesion formation in response to fungal inoculation. In contrast, concentrations of two terpenoid compounds, beta-caryophyllene and alpha-humulene, showed significant negative correlations with the lesion size. Further studies with larger sample size will help to elucidate new biomarkers or clarify the potential of the evaluated markers for use in Scots pine disease resistance breeding programs. en
dc.format.extent 15
dc.language.iso eng
dc.relation.ispartof Planta
dc.rights cc_by
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject Chemical defences
dc.subject Gene expression
dc.subject Heterobasidion
dc.subject Root rot disease
dc.subject Scots pine
dc.subject Terpenoid
dc.subject PARVIPORUM
dc.subject CONIFER
dc.subject EXPRESSION
dc.subject DEFENSES
dc.subject 1183 Plant biology, microbiology, virology
dc.subject 1182 Biochemistry, cell and molecular biology
dc.title Evaluation of potential genetic and chemical markers for Scots pine tolerance against Heterobasidion annosum infection en
dc.type Article
dc.contributor.organization Department of Forest Sciences
dc.contributor.organization Forest Ecology and Management
dc.contributor.organization Helsinki Institute of Sustainability Science (HELSUS)
dc.contributor.organization Viikki Plant Science Centre (ViPS)
dc.contributor.organization Frederick Asiegbu / Principal Investigator
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.1007/s00425-019-03270-8
dc.relation.issn 0032-0935
dc.rights.accesslevel openAccess
dc.type.version publishedVersion

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