Cell death regulation but not abscisic acid signaling is required for enhanced immunity to Botrytis in Arabidopsis cuticle-permeable mutants

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Cui , F , Wenwu , W , Wang , K , Zhang , Y , Zhubing , H , Brosche , M , Liu , S & Overmyer , K 2019 , ' Cell death regulation but not abscisic acid signaling is required for enhanced immunity to Botrytis in Arabidopsis cuticle-permeable mutants ' , Journal of Experimental Botany , vol. 70 , no. 20 , pp. 5971–5984 . https://doi.org/10.1093/jxb/erz345

Title: Cell death regulation but not abscisic acid signaling is required for enhanced immunity to Botrytis in Arabidopsis cuticle-permeable mutants
Author: Cui, Fuqiang; Wenwu, Wu; Wang, Kai; Zhang, Yuan; Zhubing, Hu; Brosche, Mikael; Liu, Shenkui; Overmyer, Kirk
Contributor: University of Helsinki, Plant-Fungal Interactions Group
University of Helsinki, Plant-Fungal Interactions Group
University of Helsinki, Plant Biology
University of Helsinki, Organismal and Evolutionary Biology Research Programme
Date: 2019-10-15
Language: eng
Number of pages: 14
Belongs to series: Journal of Experimental Botany
ISSN: 0022-0957
URI: http://hdl.handle.net/10138/308127
Abstract: Prevailing evidence indicates that abscisic acid (ABA) negatively influences immunity to the fungal pathogen Botrytis cinerea in most but not all cases. ABA is required for cuticle biosynthesis, and cuticle permeability enhances immunity to Botrytis via unknown mechanisms. This complex web of responses obscures the role of ABA in Botrytis immunity. Here, we addressed the relationships between ABA sensitivity, cuticle permeability, and Botrytis immunity in the Arabidopsis thaliana ABA-hypersensitive mutants protein phosphatase2c quadruple mutant (pp2c-q) and enhanced response to aba1 (era1-2). Neither pp2c-q nor era1-2 exhibited phenotypes predicted by the known roles of ABA; conversely, era1-2 had a permeable cuticle and was Botrytis resistant. We employed RNA-seq analysis in cuticle-permeable mutants of differing ABA sensitivities and identified a core set of constitutively activated genes involved in Botrytis immunity and susceptibility to biotrophs, independent of ABA signaling. Furthermore, botrytis susceptible1 (bos1), a mutant with deregulated cell death and enhanced ABA sensitivity, suppressed the Botrytis immunity of cuticle permeable mutants, and this effect was linearly correlated with the extent of spread of wound-induced cell death in bos1. Overall, our data demonstrate that Botrytis immunity conferred by cuticle permeability can be genetically uncoupled from PP2C-regulated ABA sensitivity, but requires negative regulation of a parallel ABA-dependent cell-death pathway.
Subject: 1183 Plant biology, microbiology, virology
BOS1
Botrytis cinerea
cell death
cuticle permeable
ERA1
farnesyl transferase
immunity
RNA sequencing
PROTEIN FARNESYLATION
TRANSCRIPTION FACTORS
GENE-EXPRESSION
PLANT IMMUNITY
RESISTANCE
DEFENSE
TOMATO
CINEREA
SUSCEPTIBILITY
RESPONSES
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