Apple B-box factors regulate light-responsive anthocyanin biosynthesis genes

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http://hdl.handle.net/10138/308131

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Plunkett , B J , Henry-Kirk , R , Friend , A , Diack , R , Helbig , S , Mouhu , K , Tomes , S , Dare , A P , Espley , R V , Putterill , J & Allan , A C 2019 , ' Apple B-box factors regulate light-responsive anthocyanin biosynthesis genes ' , Scientific Reports , vol. 9 , 17762 . https://doi.org/10.1038/s41598-019-54166-2

Title: Apple B-box factors regulate light-responsive anthocyanin biosynthesis genes
Author: Plunkett, Blue J.; Henry-Kirk, Rebecca; Friend, Adam; Diack, Robert; Helbig, Susanne; Mouhu, Katriina; Tomes, Sumathi; Dare, Andrew P.; Espley, Richard V.; Putterill, Joanna; Allan, Andrew C.
Contributor: University of Helsinki, Department of Agricultural Sciences
Date: 2019-11-28
Language: eng
Number of pages: 14
Belongs to series: Scientific Reports
ISSN: 2045-2322
URI: http://hdl.handle.net/10138/308131
Abstract: Environmentally-responsive genes can affect fruit red colour via the activation of MYB transcription factors. The apple B-box (BBX) gene, BBX33/CONSTANS-like 11 (COL11) has been reported to influence apple red-skin colour in a light- and temperature-dependent manner. To further understand the role of apple BBX genes, other members of the BBX family were examined for effects on colour regulation. Expression of 23 BBX genes in apple skin was analysed during fruit development. We investigated the diurnal rhythm of expression of the BBX genes, the anthocyanin biosynthetic genes and a MYB activator, MYB10. Transactivation assays on the MYB10 promoter, showed that BBX proteins 1, 17, 15, 35, 51, and 54 were able to directly function as activators. Using truncated versions of the MYB10 promoter, a key region was identified for activation by BBX1. BBX1 enhanced the activation of MYB10 and MdbHLH3 on the promoter of the anthocyanin biosynthetic gene DFR. In transformed apple lines, over-expression of BBX1 reduced internal ethylene content and altered both cyanidin concentration and associated gene expression. We propose that, along with environmental signals, the control of MYB10 expression by BBXs in 'Royal Gala' fruit involves the integration of the expression of multiple BBXs to regulate fruit colour.
Subject: MYB TRANSCRIPTION FACTORS
CIRCADIAN CLOCK
PROANTHOCYANIDIN BIOSYNTHESIS
FRUIT COLORATION
LOW-TEMPERATURE
RED COLORATION
ARABIDOPSIS
CONSTANS
EXPRESSION
PROTEIN
1183 Plant biology, microbiology, virology
1184 Genetics, developmental biology, physiology
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