Specific trophoblast transcripts transferred by extracellular vesicles affect gene expression in endometrial epithelial cells and may have a role in embryo-maternal crosstalk

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Es-Haghi , M , Godakumara , K , Haling , A , Lattekivi , F , Lavrits , A , Viil , J , Andronowska , A , Nafee , T , James , V , Jaakma , Ü , Salumets , A & Fazeli , A 2019 , ' Specific trophoblast transcripts transferred by extracellular vesicles affect gene expression in endometrial epithelial cells and may have a role in embryo-maternal crosstalk ' , Cell Communication and Signaling , vol. 17 , no. 1 , 146 . https://doi.org/10.1186/s12964-019-0448-x

Title: Specific trophoblast transcripts transferred by extracellular vesicles affect gene expression in endometrial epithelial cells and may have a role in embryo-maternal crosstalk
Author: Es-Haghi, Masoumeh; Godakumara, Kasun; Haling, Annika; Lattekivi, Freddy; Lavrits, Arina; Viil, Janeli; Andronowska, Aneta; Nafee, Tamer; James, Victoria; Jaakma, Ülle; Salumets, Andres; Fazeli, Alireza
Contributor: University of Helsinki, HUS Gynecology and Obstetrics
Date: 2019-11-14
Language: eng
Number of pages: 18
Belongs to series: Cell Communication and Signaling
ISSN: 1478-811X
URI: http://hdl.handle.net/10138/308163
Abstract: Background Successful establishment of pregnancy hinges on appropriate communication between the embryo and the uterus prior to implantation, but the nature of this communication remains poorly understood. Here, we tested the hypothesis that the endometrium is receptive to embryo-derived signals in the form of RNA. Methods We have utilized a non-contact co culture system to simulate the conditions of pre implantation environment of the uterus. We bioorthogonally tagged embryonic RNA and tracked the transferred transcripts to endometrium. Transferred transcripts were separated from endometrial transcripts and sequenced. Changes in endometrial transcripts were quantified using quantitative PCR. Results We show that three specific transcripts are transferred to endometrial cells. We subsequently demonstrate a role of extracellular vesicles (EVs) in this process, as EVs obtained from cultured trophoblast spheroids incubated with endometrial cells induced down-regulation of all the three identified transcripts in endometrial cells. Finally, we show that EVs/nanoparticles captured from conditioned culture media of viable embryos as opposed to degenerating embryos induce ZNF81 down-regulation in endometrial cells, hinting at the functional importance of this intercellular communication. Conclusion Ultimately, our findings demonstrate the existence of an RNA-based communication which may be of critical importance for the establishment of pregnancy.
Subject: Embryo-maternal communication
Extracellular vesicles
Non-coding RNA
HUMAN CUMULUS CELLS
IN-VITRO
INTERCELLULAR COMMUNICATION
NONCODING RNAS
HUMAN BLASTOCYST
UNIQUE FEATURES
DOWN-REGULATION
IMPLANTATION
PROTEINS
DIFFERENTIATION
1182 Biochemistry, cell and molecular biology
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