Allogenic Faecal Microbiota Transfer Induces Immune-Related Gene Sets in the Colon Mucosa of Patients with Irritable Bowel Syndrome

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Holster , S , Hooiveld , G J , Repsilber , D , de Vos , W M , Brummer , R J & König , J 2019 , ' Allogenic Faecal Microbiota Transfer Induces Immune-Related Gene Sets in the Colon Mucosa of Patients with Irritable Bowel Syndrome ' , Biomolecules , vol. 9 , no. 10 , 586 . https://doi.org/10.3390/biom9100586

Title: Allogenic Faecal Microbiota Transfer Induces Immune-Related Gene Sets in the Colon Mucosa of Patients with Irritable Bowel Syndrome
Author: Holster, Savanne; Hooiveld, Guido J.; Repsilber, Dirk; de Vos, Willem M.; Brummer, Robert J.; König, Julia
Contributor: University of Helsinki, Medicum
Date: 2019-10
Language: eng
Number of pages: 18
Belongs to series: Biomolecules
ISSN: 2218-273X
URI: http://hdl.handle.net/10138/308172
Abstract: Faecal microbiota transfer (FMT) consists of the introduction of new microbial communities into the intestine of a patient, with the aim of restoring a disturbed gut microbiota. Even though it is used as a potential treatment for various diseases, it is unknown how the host mucosa responds to FMT. This study aims to investigate the colonic mucosa gene expression response to allogenic (from a donor) or autologous (own) FMT in patients with irritable bowel syndrome (IBS). In a recently conducted randomised, double-blinded, controlled clinical study, 17 IBS patients were treated with FMT by colonoscopy. RNA was isolated from colonic biopsies collected by sigmoidoscopy at baseline, as well as two weeks and eight weeks after FMT. In patients treated with allogenic FMT, predominantly immune response-related gene sets were induced, with the strongest response two weeks after the FMT. In patients treated with autologous FMT, predominantly metabolism-related gene sets were affected. Furthermore, several microbiota genera showed correlations with immune-related gene sets, with different correlations found after allogenic compared to autologous FMT. This study shows that the microbe-host response is influenced by FMT on the mucosal gene expression level, and that there are clear differences in response to allogenic compared to autologous FMT.
Subject: faecal microbiota transplantation
irritable bowel syndrome
gene expression
microbiota
host-microbe interaction
ACTIVE ULCERATIVE-COLITIS
INTESTINAL MICROBIOTA
INSULIN SENSITIVITY
TRANSPLANTATION
PROBIOTICS
EXPRESSION
PATHWAYS
PLACEBO
1182 Biochemistry, cell and molecular biology
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