On-line profiling of volatile compounds produced in vitro by pathogenic oral bacteria

Show full item record



Permalink

http://hdl.handle.net/10138/308438

Citation

Roslund , K , Lehto , M , Pussinen , P , Groop , P-H , Halonen , L & Metsälä , M 2020 , ' On-line profiling of volatile compounds produced in vitro by pathogenic oral bacteria ' , Journal of Breath Research , vol. 14 , no. 1 , 016010 . https://doi.org/10.1088/1752-7163/ab5559 , https://doi.org/10.1088/1752-7163/ab5559

Title: On-line profiling of volatile compounds produced in vitro by pathogenic oral bacteria
Author: Roslund, Kajsa; Lehto, Markku; Pussinen, Pirkko; Groop, Per-Henrik; Halonen, Lauri; Metsälä, Markus
Contributor: University of Helsinki, Department of Chemistry
University of Helsinki, HUS Abdominal Center
University of Helsinki, Department of Oral and Maxillofacial Diseases
University of Helsinki, HUS Abdominal Center
University of Helsinki, Department of Chemistry
University of Helsinki, University Management
Date: 2020
Language: eng
Number of pages: 15
Belongs to series: Journal of Breath Research
ISSN: 1752-7155
URI: http://hdl.handle.net/10138/308438
Abstract: Infections by oral pathogens are one of the most common health problems worldwide. Due to the intimate connection between exhaled breath and the oral cavity, breath analysis could potentially be used to diagnose these infections. However, little is known about the volatile emissions of important oral pathogens that are connected with gingivitis and periodontitis. In this study, we have performed in vitro headspace measurements on four important oral pathogens (P. gingivalis, T. forsythia, P. intermedia and P. nigrescens) using proton transfer reaction time-of-flight mass spectrometry (PTR-TOF-MS). Some of the most abundant compounds produced by the bacteria include hydrogen sulphide, methanethiol, acetone, dimethylsulphide, isoprene, cyclopentanone and indole as tentatively assigned from the mass spectra. Several other abundant mass signals were recorded but the assignment of these is less certain. Some of the bacterial species can be separated from each other by the emitted volatile fingerprints. The results of this study can be used in potential development of a diagnostic breath test for oral infections. In addition, as several of the measured compounds are known to be toxic, the results point to an intriguing possibility of studying the connection between the bacterial virulence and the emitted volatile compounds.
Subject: 3121 General medicine, internal medicine and other clinical medicine
1182 Biochemistry, cell and molecular biology
Rights:


Files in this item

Total number of downloads: Loading...

Files Size Format View
Roslund_2020_J._Breath_Res._14_016010.pdf 1.458Mb PDF View/Open

This item appears in the following Collection(s)

Show full item record