On-line profiling of volatile compounds produced in vitro by pathogenic oral bacteria

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Roslund , K , Lehto , M , Pussinen , P , Groop , P-H , Halonen , L & Metsälä , M 2020 , ' On-line profiling of volatile compounds produced in vitro by pathogenic oral bacteria ' , Journal of Breath Research , vol. 14 , no. 1 , 016010 . https://doi.org/10.1088/1752-7163/ab5559 , https://doi.org/10.1088/1752-7163/ab5559

Title: On-line profiling of volatile compounds produced in vitro by pathogenic oral bacteria
Author: Roslund, Kajsa; Lehto, Markku; Pussinen, Pirkko; Groop, Per-Henrik; Halonen, Lauri; Metsälä, Markus
Contributor organization: Department of Chemistry
University of Helsinki
HUS Abdominal Center
Nefrologian yksikkö
Research Programs Unit
CAMM - Research Program for Clinical and Molecular Metabolism
Department of Oral and Maxillofacial Diseases
University Management
Department of Medicine
Per Henrik Groop / Principal Investigator
Molecular Science
Date: 2020
Language: eng
Number of pages: 15
Belongs to series: Journal of Breath Research
ISSN: 1752-7155
DOI: https://doi.org/10.1088/1752-7163/ab5559
URI: http://hdl.handle.net/10138/308438
Abstract: Infections by oral pathogens are one of the most common health problems worldwide. Due to the intimate connection between exhaled breath and the oral cavity, breath analysis could potentially be used to diagnose these infections. However, little is known about the volatile emissions of important oral pathogens that are connected with gingivitis and periodontitis. In this study, we have performed in vitro headspace measurements on four important oral pathogens (P. gingivalis, T. forsythia, P. intermedia and P. nigrescens) using proton transfer reaction time-of-flight mass spectrometry (PTR-TOF-MS). Some of the most abundant compounds produced by the bacteria include hydrogen sulphide, methanethiol, acetone, dimethylsulphide, isoprene, cyclopentanone and indole as tentatively assigned from the mass spectra. Several other abundant mass signals were recorded but the assignment of these is less certain. Some of the bacterial species can be separated from each other by the emitted volatile fingerprints. The results of this study can be used in potential development of a diagnostic breath test for oral infections. In addition, as several of the measured compounds are known to be toxic, the results point to an intriguing possibility of studying the connection between the bacterial virulence and the emitted volatile compounds.
Subject: 3121 General medicine, internal medicine and other clinical medicine
1182 Biochemistry, cell and molecular biology
Peer reviewed: Yes
Rights: cc_by
Usage restriction: openAccess
Self-archived version: publishedVersion

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