On-line profiling of volatile compounds produced in vitro by pathogenic oral bacteria

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dc.contributor.author Roslund, Kajsa
dc.contributor.author Lehto, Markku
dc.contributor.author Pussinen, Pirkko
dc.contributor.author Groop, Per-Henrik
dc.contributor.author Halonen, Lauri
dc.contributor.author Metsälä, Markus
dc.date.accessioned 2019-12-17T09:51:01Z
dc.date.available 2019-12-17T09:51:01Z
dc.date.issued 2020
dc.identifier.citation Roslund , K , Lehto , M , Pussinen , P , Groop , P-H , Halonen , L & Metsälä , M 2020 , ' On-line profiling of volatile compounds produced in vitro by pathogenic oral bacteria ' , Journal of Breath Research , vol. 14 , no. 1 , 016010 . https://doi.org/10.1088/1752-7163/ab5559 , https://doi.org/10.1088/1752-7163/ab5559
dc.identifier.other PURE: 128543183
dc.identifier.other PURE UUID: dfd85f54-2b51-4e66-a31a-00e0b1c734a9
dc.identifier.other ORCID: /0000-0002-0716-7132/work/66366156
dc.identifier.other ORCID: /0000-0002-2271-611X/work/66367143
dc.identifier.other ORCID: /0000-0003-3563-1876/work/95369436
dc.identifier.other WOS: 000744965100002
dc.identifier.uri http://hdl.handle.net/10138/308438
dc.description.abstract Infections by oral pathogens are one of the most common health problems worldwide. Due to the intimate connection between exhaled breath and the oral cavity, breath analysis could potentially be used to diagnose these infections. However, little is known about the volatile emissions of important oral pathogens that are connected with gingivitis and periodontitis. In this study, we have performed in vitro headspace measurements on four important oral pathogens (P. gingivalis, T. forsythia, P. intermedia and P. nigrescens) using proton transfer reaction time-of-flight mass spectrometry (PTR-TOF-MS). Some of the most abundant compounds produced by the bacteria include hydrogen sulphide, methanethiol, acetone, dimethylsulphide, isoprene, cyclopentanone and indole as tentatively assigned from the mass spectra. Several other abundant mass signals were recorded but the assignment of these is less certain. Some of the bacterial species can be separated from each other by the emitted volatile fingerprints. The results of this study can be used in potential development of a diagnostic breath test for oral infections. In addition, as several of the measured compounds are known to be toxic, the results point to an intriguing possibility of studying the connection between the bacterial virulence and the emitted volatile compounds. en
dc.format.extent 15
dc.language.iso eng
dc.relation.ispartof Journal of Breath Research
dc.rights cc_by
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject 3121 General medicine, internal medicine and other clinical medicine
dc.subject 1182 Biochemistry, cell and molecular biology
dc.title On-line profiling of volatile compounds produced in vitro by pathogenic oral bacteria en
dc.type Article
dc.contributor.organization Department of Chemistry
dc.contributor.organization University of Helsinki
dc.contributor.organization HUS Abdominal Center
dc.contributor.organization Nefrologian yksikkö
dc.contributor.organization Research Programs Unit
dc.contributor.organization CAMM - Research Program for Clinical and Molecular Metabolism
dc.contributor.organization Department of Oral and Maxillofacial Diseases
dc.contributor.organization University Management
dc.contributor.organization Department of Medicine
dc.contributor.organization Per Henrik Groop / Principal Investigator
dc.contributor.organization Molecular Science
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.1088/1752-7163/ab5559
dc.relation.issn 1752-7155
dc.rights.accesslevel openAccess
dc.type.version publishedVersion

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