Lumen, D.; Näkki, S.; Imlimthan, S.; Lambidis, E.; Sarparanta, M.; Xu, W.; Lehto, V.-P.; Airaksinen, A.J. Site-Specific 111In-Radiolabeling of Dual-PEGylated Porous Silicon Nanoparticles and Their In Vivo Evaluation in Murine 4T1 Breast Cancer Model. Pharmaceutics 2019, 11, 686.
Title: | Site-Specific 111In-Radiolabeling of Dual-PEGylated Porous Silicon Nanoparticles and Their In Vivo Evaluation in Murine 4T1 Breast Cancer Model |
Author: | Lumen, Dave; Näkki, Simo; Imlimthan, Surachet; Lambidis, Elisavet; Sarparanta, Mirkka; Xu, Wujun; Lehto, Vesa-Pekka; Airaksinen, Anu J. |
Publisher: | Multidisciplinary Digital Publishing Institute |
Date: | 2019-12-17 |
URI: | http://hdl.handle.net/10138/308693 |
Abstract: | Polyethylene glycol (PEG) has been successfully used for improving circulation time of several nanomaterials but prolonging the circulation of porous silicon nanoparticles (PSi NPs) has remained challenging. Here, we report a site specific radiolabeling of dual-PEGylated thermally oxidized porous silicon (DPEG-TOPSi) NPs and investigation of influence of the PEGylation on blood circulation time of TOPSi NPs. <i>Trans</i>-cyclooctene conjugated DPEG-TOPSi NPs were radiolabeled through a click reaction with [<sup>111</sup>In]In-DOTA-PEG<sub>4</sub>-tetrazine (DOTA = 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid) and the particle behavior was evaluated in vivo in Balb/c mice bearing 4T1 murine breast cancer allografts. The dual-PEGylation significantly prolonged circulation of [<sup>111</sup>In]In-DPEG-TOPSi particles when compared to non-PEGylated control particles, yielding 10.8 ± 1.7% of the injected activity/g in blood at 15 min for [<sup>111</sup>In]In-DPEG-TOPSi NPs. The improved circulation time will be beneficial for the accumulation of targeted DPEG-TOPSi to tumors. |
Total number of downloads: Loading...
Files | Size | Format | View |
---|---|---|---|
pharmaceutics-11-00686.pdf | 3.124Mb |
View/ |