TRITEX: chromosome-scale sequence assembly of Triticeae genomes with open-source tools

Show simple item record Monat, Cécile Padmarasu, Sudharsan Lux, Thomas Wicker, Thomas Gundlach, Heidrun Himmelbach, Axel Ens, Jennifer Li, Chengdao Muehlbauer, Gary J Schulman, Alan H. Waugh, Robbie Braumann, Ilka Pozniak, Curtis Scholz, Uwe Mayer, Klaus F X Spannagl, Manuel Stein, Nils Mascher, Martin 2019-12-22T12:28:39Z 2019-12-22T12:28:39Z 2019-12-18
dc.identifier.citation Genome Biology. 2019 Dec 18;20(1):284
dc.description.abstract Abstract Chromosome-scale genome sequence assemblies underpin pan-genomic studies. Recent genome assembly efforts in the large-genome Triticeae crops wheat and barley have relied on the commercial closed-source assembly algorithm DeNovoMagic. We present TRITEX, an open-source computational workflow that combines paired-end, mate-pair, 10X Genomics linked-read with chromosome conformation capture sequencing data to construct sequence scaffolds with megabase-scale contiguity ordered into chromosomal pseudomolecules. We evaluate the performance of TRITEX on publicly available sequence data of tetraploid wild emmer and hexaploid bread wheat, and construct an improved annotated reference genome sequence assembly of the barley cultivar Morex as a community resource.
dc.language.iso en
dc.publisher BioMed Central
dc.title TRITEX: chromosome-scale sequence assembly of Triticeae genomes with open-source tools en 2019-12-22T12:28:39Z
dc.rights.holder The Author(s).

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