TRITEX: chromosome-scale sequence assembly of Triticeae genomes with open-source tools

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dc.contributor.author Monat, Cécile
dc.contributor.author Padmarasu, Sudharsan
dc.contributor.author Lux, Thomas
dc.contributor.author Wicker, Thomas
dc.contributor.author Gundlach, Heidrun
dc.contributor.author Himmelbach, Axel
dc.contributor.author Ens, Jennifer
dc.contributor.author Li, Chengdao
dc.contributor.author Muehlbauer, Gary J
dc.contributor.author Schulman, Alan H.
dc.contributor.author Waugh, Robbie
dc.contributor.author Braumann, Ilka
dc.contributor.author Pozniak, Curtis
dc.contributor.author Scholz, Uwe
dc.contributor.author Mayer, Klaus F X
dc.contributor.author Spannagl, Manuel
dc.contributor.author Stein, Nils
dc.contributor.author Mascher, Martin
dc.date.accessioned 2019-12-22T12:28:39Z
dc.date.available 2019-12-22T12:28:39Z
dc.date.issued 2019-12-18
dc.identifier.citation Genome Biology. 2019 Dec 18;20(1):284
dc.identifier.uri http://hdl.handle.net/10138/308716
dc.description.abstract Abstract Chromosome-scale genome sequence assemblies underpin pan-genomic studies. Recent genome assembly efforts in the large-genome Triticeae crops wheat and barley have relied on the commercial closed-source assembly algorithm DeNovoMagic. We present TRITEX, an open-source computational workflow that combines paired-end, mate-pair, 10X Genomics linked-read with chromosome conformation capture sequencing data to construct sequence scaffolds with megabase-scale contiguity ordered into chromosomal pseudomolecules. We evaluate the performance of TRITEX on publicly available sequence data of tetraploid wild emmer and hexaploid bread wheat, and construct an improved annotated reference genome sequence assembly of the barley cultivar Morex as a community resource.
dc.language.iso en
dc.publisher BioMed Central
dc.title TRITEX: chromosome-scale sequence assembly of Triticeae genomes with open-source tools en
dc.date.updated 2019-12-22T12:28:39Z
dc.rights.holder The Author(s).
dc.type.uri http://purl.org/eprint/entityType/ScholarlyWork
dc.type.uri http://purl.org/eprint/entityType/Expression
dc.type.uri http://purl.org/eprint/type/JournalArticle

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