Design and validation of an STR hexaplex assay for DNA profiling of grapevine cultivars

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Drabek , J , Smolikova , M , Kalendar , R , Pinto , F A L , Pavlousek , P , KleparnIk , K & Frebort , I 2016 , ' Design and validation of an STR hexaplex assay for DNA profiling of grapevine cultivars ' , Electrophoresis , vol. 37 , no. 23-24 , pp. 3059-3067 . https://doi.org/10.1002/elps.201600068

Title: Design and validation of an STR hexaplex assay for DNA profiling of grapevine cultivars
Author: Drabek, Jiri; Smolikova, Michaela; Kalendar, Ruslan; Pinto, Fernando A. Lopes; Pavlousek, Pavel; KleparnIk, Karel; Frebort, Ivo
Other contributor: University of Helsinki, Institute of Biotechnology
Date: 2016-12
Language: eng
Number of pages: 9
Belongs to series: Electrophoresis
ISSN: 0173-0835
DOI: https://doi.org/10.1002/elps.201600068
URI: http://hdl.handle.net/10138/308718
Abstract: Although the analysis of length polymorphism at STR loci has become a method of choice for grape cultivar identification, the standardization of methods for this purpose lags behind that of methods for DNA profiling in human and animal forensic genetics. The aim of this study was thus to design and validate a grapevine STR protocol with a practically useful level of multiplexing. Using free bioinformatics tools, published primer sequences, and nucleotide databases, we constructed and optimized a primer set for the simultaneous analysis of six STR loci (VVIi51, scu08vv, scu05vv, VVMD17, VrZAG47, and VrZAG83) by multiplex PCR and CE with laser-induced fluorescence, and tested it on 90 grape cultivars. The new protocol requires subnanogram quantities of the DNA template and enables automated, high-throughput genetic analysis with reasonable discriminatory power. As such, it represents a step toward further standardization of grape DNA profiling.
Subject: Grapevine DNA analysis
Multiplex PCR
STRs
Vitis vinifera L
VITIS-VINIFERA L.
POLYMERASE-CHAIN-REACTION
MICROSATELLITE MARKERS
MOLECULAR TOOLS
GENETIC-MARKERS
MULTIPLEX PCR
IDENTIFICATION
WINES
SET
DIFFERENTIATION
1182 Biochemistry, cell and molecular biology
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