Near-Infrared Fluorescent Proteins : Multiplexing and Optogenetics across Scales

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Shcherbakova , D M , Stepanenko , O V , Turoverov , K K & Verkhusha , V V 2018 , ' Near-Infrared Fluorescent Proteins : Multiplexing and Optogenetics across Scales ' , Trends in Biotechnology , vol. 36 , no. 12 , pp. 1230-1243 . https://doi.org/10.1016/j.tibtech.2018.06.011

Title: Near-Infrared Fluorescent Proteins : Multiplexing and Optogenetics across Scales
Author: Shcherbakova, Daria M.; Stepanenko, Olesya V.; Turoverov, Konstantin K.; Verkhusha, Vladislav V.
Contributor: University of Helsinki, Department of Biochemistry and Developmental Biology
Date: 2018-12
Language: eng
Number of pages: 14
Belongs to series: Trends in Biotechnology
ISSN: 0167-7799
URI: http://hdl.handle.net/10138/308989
Abstract: Since mammalian tissue is relatively transparent to near-infrared (NIR) light, NIR fluorescentproteins(FPs) engineeredfrombacterialphytochromeshave become widely used probes for non-invasive in vivo imaging. Recently, these genetically encoded NIR probes have been substantially improved, enabling imaging experiments that were not possible previously. Here, we discuss the use of monomeric NIR FPs and NIR biosensors for multiplexed imaging with common visible GFP-based probes and blue light-activatable optogenetic tools. These NIR probes are suitable for visualization of functional activities from molecular to organismal levels. In combination with advanced imaging techniques, such as two-photon microscopy with adaptive optics, photoacoustic tomography and its recent modification reversibly switchable photoacoustic computed tomography, NIR probes allow subcellular resolution at millimeter depths.
Subject: SPATIOTEMPORAL DYNAMICS
REPORTER
EXCITATION
RED
PHYTOCHROME
BRIGHT
IRFP
TOMOGRAPHY
MICROSCOPY
BIOSENSORS
3111 Biomedicine
318 Medical biotechnology
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