Metabarcoding successfully tracks temporal changes in eukaryotic communities in coastal sediments

Show full item record



Permalink

http://hdl.handle.net/10138/309061

Citation

Salonen , I S , Chronopoulou , P-M , Leskinen , E & Koho , K A 2019 , ' Metabarcoding successfully tracks temporal changes in eukaryotic communities in coastal sediments ' , FEMS Microbiology Ecology , vol. 95 , no. 1 , fiy226 . https://doi.org/10.1093/femsec/fiy226

Title: Metabarcoding successfully tracks temporal changes in eukaryotic communities in coastal sediments
Author: Salonen, I. S.; Chronopoulou, P.-M.; Leskinen, E.; Koho, K. A.
Contributor: University of Helsinki, Helsinki Institute of Sustainability Science (HELSUS)
University of Helsinki, Ecosystems and Environment Research Programme
University of Helsinki, Ecosystems and Environment Research Programme
University of Helsinki, Ecosystems and Environment Research Programme
Date: 2019-01
Language: eng
Number of pages: 11
Belongs to series: FEMS Microbiology Ecology
ISSN: 0168-6496
URI: http://hdl.handle.net/10138/309061
Abstract: Metabarcoding is a method that combines high-throughput DNA sequencing and DNA-based identification. Previously, this method has been successfully used to target spatial variation of eukaryote communities in marine sediments, however, the temporal changes in these communities remain understudied. Here, we follow the temporal changes of the eukaryote communities in Baltic Sea surface sediments collected from two coastal localities during three seasons of two consecutive years. Our study reveals that the structure of the sediment eukaryotic ecosystem was primarily driven by annual and seasonal changes in prevailing environmental conditions, whereas spatial variation was a less significant factor in explaining the variance in eukaryotic communities over time. Therefore, our data suggests that shifts in regional climate regime or large-scale changes in the environment are the overdriving factors in shaping the coastal eukaryotic sediment ecosystems rather than small-scale changes in local environmental conditions or heterogeneity in ecosystem structure. More studies targeting temporal changes are needed to further understand the long-term trends in ecosystem stability and response to climate change. Furthermore, this work contributes to the recent efforts in developing metabarcoding applications for environmental biomonitoring, proving a comprehensive option for traditional monitoring approaches.
Subject: 18S rRNA gene
BALTIC SEA
DIVERSITY
V4
V9 REGIONS
metabarcoding
next-generation sequencing
sediment
temporal variation
1181 Ecology, evolutionary biology
1183 Plant biology, microbiology, virology
Rights:


Files in this item

Total number of downloads: Loading...

Files Size Format View
ISalonen.pdf 72.42Kb PDF View/Open

This item appears in the following Collection(s)

Show full item record