Field-flow fractionation of cationic cellulose derivatives

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Pysyväisosoite

http://hdl.handle.net/10138/309185

Lähdeviite

Tenkanen , M & Pitkänen , L 2019 , ' Field-flow fractionation of cationic cellulose derivatives ' , Chromatographia , vol. 82 , no. 12 , pp. 1827-1832 . https://doi.org/10.1007/s10337-019-03800-2

Julkaisun nimi: Field-flow fractionation of cationic cellulose derivatives
Tekijä: Tenkanen, Maija; Pitkänen, Leena
Muu tekijä: University of Helsinki, Department of Food and Nutrition
University of Helsinki, Department of Food and Nutrition
Päiväys: 2019-12
Kieli: eng
Sivumäärä: 6
Kuuluu julkaisusarjaan: Chromatographia
ISSN: 0009-5893
URI: http://hdl.handle.net/10138/309185
Tiivistelmä: The asymmetric flow field-flow fractionation (AF4) method was developed for cationic cellulose derivatives. AF4 is the method of choice especially for high-molar mass samples, which are challenging to characterize with conventional chromatographic techniques such as size-exclusion chromatography (SEC). The cationic charge of macromolecules also complicates the size-based separations where no interaction between the analytes and the column stationary phase (SEC) or membrane (AF4) should occur. However, many column matrices and membranes carry negative charge and thus preventing interactions between cationic analytes and negatively charged separation support should be taken into consideration when doing method development. In this study, two eluent compositions, neutral and acidic, were tested for AF4 separation of cationic hydroxyethyl celluloses with varying charge densities. The eluent composition with a pH below the isoelectric point of regenerated cellulose membrane, which was used in this AF4 study, enabled the size-based separation with close to 100% analysis recovery. Macromolecular parameters (molar mass and radius of gyration) and conformation were investigated by coupling a multi-angle light scattering detector and differential refractometer to the AF4 system.
Avainsanat: 416 Food Science
116 Chemical sciences
Field-flow fractionation
Separation
Cationic polysaccharides
Charge density
POLYMERS
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