Metabolic Remodeling during Long-Lasting Cultivation of the Endomyces magnusii Yeast on Oxidative and Fermentative Substrates

Show simple item record

dc.contributor.author Isakova, Elena P.
dc.contributor.author Matushkina, Irina N.
dc.contributor.author Popova, Tatyana N.
dc.contributor.author Dergacheva, Darya I.
dc.contributor.author Gessler, Natalya N.
dc.contributor.author Klein, Olga I.
dc.contributor.author Semenikhina, Anastasya V.
dc.contributor.author Deryabina, Yulia I.
dc.contributor.author La Porta, Nicola
dc.contributor.author Saris, Nils-Eric L.
dc.date.accessioned 2020-01-10T09:02:23Z
dc.date.available 2020-01-10T09:02:23Z
dc.date.issued 2020-01-09
dc.identifier.citation Isakova, E.P.; Matushkina, I.N.; Popova, T.N.; Dergacheva, D.I.; Gessler, N.N.; Klein, O.I.; Semenikhina, A.V.; Deryabina, Y.I.; La Porta, N.; Saris, N.-E.L. Metabolic Remodeling during Long-Lasting Cultivation of the Endomyces magnusii Yeast on Oxidative and Fermentative Substrates. Microorganisms 2020, 8, 91.
dc.identifier.uri http://hdl.handle.net/10138/309219
dc.description.abstract In this study, we evaluated the metabolic profile of the aerobic microorganism of <i>Endomyces magnusii</i> with a complete respiration chain and well-developed mitochondria system during long-lasting cultivation. The yeast was grown in batches using glycerol and glucose as the sole carbon source for a week. The profile included the cellular biological and chemical parameters, which determined the redox status of the yeast cells. We studied the activities of the antioxidant systems (catalases and superoxide dismutases), glutathione system enzymes (glutathione peroxidase and reductase), aconitase, as well as the main enzymes maintaining NADPH levels in the cells (glucose-6-phosphate dehydrogenase and NADP<sup>+</sup>-isocitrate dehydrogenase) during aging of <i>Endomyces magnusii</i> on two kinds of substrates. We also investigated the dynamics of change in oxidized and reduced glutathione, conjugated dienes, and reactive oxidative species in the cells at different growth stages, including the deep stationary stages. Our results revealed a similar trend in the changes in the activity of all the enzymes tested, which increased 2&ndash;4-fold upon aging. The yeast cytosol had a very high reduced glutathione content, 22 times than that of <i>Saccharomyces cerevisiae</i>, and remained unchanged during growth, whereas there was a 7.5-fold increase in the reduced glutathione-to-oxidized glutathione ratio. The much higher level of reactive oxidative species was observed in the cells in the late and deep stationary phases, especially in the cells using glycerol. Cell aging of the culture grown on glycerol, which promotes active oxidative phosphorylation in the mitochondria, facilitated the functioning of powerful antioxidant systems (catalases, superoxide dismutases, and glutathione system enzymes) induced by reactive oxidative species. Moreover, it stimulated NADPH synthesis, regulating the cytosolic reduced glutathione level, which in turn determines the redox potential of the yeast cell during the early aging process.
dc.publisher Multidisciplinary Digital Publishing Institute
dc.title Metabolic Remodeling during Long-Lasting Cultivation of the Endomyces magnusii Yeast on Oxidative and Fermentative Substrates
dc.date.updated 2020-01-10T09:02:24Z
dc.type.uri http://purl.org/eprint/entityType/JournalArticle
dc.type.uri http://purl.org/eprint/entityType/Expression
dc.type.uri http://purl.org/eprint/entityType/Expression

Files in this item

Total number of downloads: Loading...

Files Size Format View
microorganisms-08-00091.pdf 3.509Mb PDF View/Open

This item appears in the following Collection(s)

Show simple item record