Simultaneous electrochemical detection of tramadol and O-desmethyltramadol with Nafion-coated tetrahedral amorphous carbon electrode

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Mynttinen , E , Wester , N , Lilius , T , Kalso , E , Koskinen , J & Laurila , T 2019 , ' Simultaneous electrochemical detection of tramadol and O-desmethyltramadol with Nafion-coated tetrahedral amorphous carbon electrode ' , Electrochimica Acta , vol. 295 , pp. 347-353 . https://doi.org/10.1016/j.electacta.2018.10.148

Title: Simultaneous electrochemical detection of tramadol and O-desmethyltramadol with Nafion-coated tetrahedral amorphous carbon electrode
Author: Mynttinen, Elsi; Wester, Niklas; Lilius, Tuomas; Kalso, Eija; Koskinen, Jari; Laurila, Tomi
Contributor: University of Helsinki, Medicum
University of Helsinki, Eija Kalso / Principal Investigator
Date: 2019-02-01
Language: eng
Number of pages: 7
Belongs to series: Electrochimica Acta
ISSN: 0013-4686
URI: http://hdl.handle.net/10138/309283
Abstract: Tramadol (TR) is a member of the opioid family and is widely used for pain treatment in clinical patient care. The analgesic effect of tramadol is induced primarily by its main metabolite Odesmethyltramadol (ODMT). Due to interindividual differences in the TR metabolism to ODMT, the responses to TR vary highly between patients. Thus, a fast and selective method for simultaneous detection of TR and ODMT would increase the patient safety and pain treatment efficacy. In this study, a tetrahedral amorphous carbon (ta-C) electrode coated with a thin dip-coated recast Nafion membrane was fabricated for selective electrochemical determination of TR and ODMT. With this Nafion/ta-C electrode, simultaneous detection of TR and ODMT was achieved with linear ranges of 1-12.5 mu M and 1-15 mu M, respectively. The limits of detection were 131 nM for TR and 209 nM for ODMT. Both analytes were also measured in the presence of several common interferents, demonstrating the high selectivity of the fabricated electrode. In addition, the effect of pH on the peak potential was studied to observe the electrochemical behavior of the analytes at the electrode. Finally, clinically relevant concentrations of TR and ODMT were simultaneously detected from diluted human plasma to assess the applicability of the electrode in real samples. The fabricated Nafion/ta-C electrode was found successful in the simultaneous electrochemical detection of TR and ODMT in both buffer solution and in human plasma. (C) 2018 Elsevier Ltd. All rights reserved.
Subject: Tetrahedral amorphous carbon
Nafion
Tramadol
O-desmethyltramadol
Electrochemical sensor
DIAMOND-LIKE CARBON
MOLECULARLY IMPRINTED POLYMER
VOLTAMMETRIC DETERMINATION
SENSITIVE DETERMINATION
SENSOR
FILMS
PARACETAMOL
DOPAMINE
NANOPARTICLES
FABRICATION
3111 Biomedicine
3112 Neurosciences
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