Discovery of increased epidermal DNAH10 expression after regeneration of dermis in a randomized with-in person trial - reflections on psoriatic inflammation

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Lagus , H , Klaas , M , Juteau , S , Elomaa , O , Kere , J , Vuola , J , Jaks , V & Kankuri , E 2019 , ' Discovery of increased epidermal DNAH10 expression after regeneration of dermis in a randomized with-in person trial - reflections on psoriatic inflammation ' , Scientific Reports , vol. 9 , 19136 . https://doi.org/10.1038/s41598-019-53874-z

Title: Discovery of increased epidermal DNAH10 expression after regeneration of dermis in a randomized with-in person trial - reflections on psoriatic inflammation
Author: Lagus, Heli; Klaas, Mariliis; Juteau, Susanna; Elomaa, Outi; Kere, Juha; Vuola, Jyrki; Jaks, Viljar; Kankuri, Esko
Contributor: University of Helsinki, HUS Musculoskeletal and Plastic Surgery
University of Helsinki, HUSLAB
University of Helsinki, STEMM - Stem Cells and Metabolism Research Program
University of Helsinki, Karolinska Institutet
University of Helsinki, HUS Musculoskeletal and Plastic Surgery
University of Helsinki, University Management
Date: 2019-12-13
Language: eng
Number of pages: 11
Belongs to series: Scientific Reports
ISSN: 2045-2322
URI: http://hdl.handle.net/10138/309543
Abstract: Because molecular memories of past inflammatory events can persist in epidermal cells, we evaluated the long-term epidermal protein expression landscapes after dermal regeneration and in psoriatic inflammation. We first characterized the effects of two dermal regeneration strategies on transplants of indicator split-thickness skin grafts (STSGs) in ten adult patients with deep burns covering more than 20% of their body surface area. After fascial excision, three adjacent areas within the wound were randomized to receive a permanent dermal matrix, a temporary granulation-tissue-inducing dressing or no dermal component as control. Control areas were covered with STSG immediately, and treated areas after two-weeks of dermis formation. Epidermis-dermis-targeted proteomics of one-year-follow-up samples were performed for protein expression profiling. Epidermal expression of axonemal dynein heavy chain 10 (DNAH10) was increased 20-fold in samples having had regenerating dermis vs control. Given the dermal inflammatory component found in our dermal regeneration samples as well as in early psoriatic lesions, we hypothesized that DNAH10 protein expression also would be affected in psoriatic skin samples. We discovered increased DNAH10 expression in inflammatory lesions when compared to unaffected skin. Our results associate DNAH10 expression with cell proliferation and inflammation as well as with the epidermal memory resulting from the previous regenerative signals of dermis. This study (ISRCTN14499986) was funded by the Finnish Ministry of Defense and by government subsidies for medical research.
Subject: MESSENGER-RNA DECAY
PRIMARY CILIUM
STEM-CELLS
TNF-ALPHA
T-CELLS
SKIN
HOMEOSTASIS
BARRIER
DIFFERENTIATION
TISSUE
3111 Biomedicine
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