Multi-parametric surface plasmon resonance platform for studying liposome-serum interactions and protein corona formation

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Kari , O K , Rojalin , T , Salmaso , S , Barattin , M , Jarva , H , Meri , S , Yliperttula , M , Viitala , T & Urtti , A 2017 , ' Multi-parametric surface plasmon resonance platform for studying liposome-serum interactions and protein corona formation ' , Drug Delivery and Translational Research , vol. 7 , no. 2 , pp. 1-13 . https://doi.org/10.1007/s13346-016-0320-0

Title: Multi-parametric surface plasmon resonance platform for studying liposome-serum interactions and protein corona formation
Author: Kari, Otto K.; Rojalin, Tatu; Salmaso, Stefano; Barattin, Michela; Jarva, Hanna; Meri, Seppo; Yliperttula, Marjo; Viitala, Tapani; Urtti, Arto
Contributor organization: Faculty of Pharmacy
Drug Delivery Unit
HUSLAB
Research Programs Unit
Immunobiology Research Program
Department of Bacteriology and Immunology
Medicum
Clinicum
Seppo Meri / Principal Investigator
Division of Pharmaceutical Biosciences
Nanobio Pharmaceutics
Drug Research Program
Pharmaceutical biophysics group
Biopharmaceutics Group
Date: 2017
Language: eng
Number of pages: 13
Belongs to series: Drug Delivery and Translational Research
ISSN: 2190-393X
DOI: https://doi.org/10.1007/s13346-016-0320-0
URI: http://hdl.handle.net/10138/309640
Abstract: When nanocarriers are administered into the blood circulation, a complex biomolecular layer known as the “protein corona” associates with their surface. Although the drivers of corona formation are not known, it is widely accepted that this layer mediates biological interactions of the nanocarrier with its surroundings. Label-free optical methods can be used to study protein corona formation without interfering with its dynamics. We demonstrate the proof-of-concept for a multi-parametric surface plasmon resonance (MP-SPR) technique in monitoring the formation of a protein corona on surface-immobilized liposomes subjected to flowing 100 % human serum. We observed the formation of formulation-dependent “hard” and “soft” coronas with distinct refractive indices, layer thicknesses, and surface mass densities. MP-SPR was also employed to determine the affinity (KD) of a complement system molecule (C3b) with cationic liposomes with and without polyethylene glycol. Tendency to create a thick corona correlated with a higher affinity of opsonin C3b for the surface. The label-free platform provides a fast and robust preclinical tool for tuning nanocarrier surface architecture and composition to control protein corona formation.When nanocarriers are administered into the blood circulation, a complex biomolecular layer known as the "protein corona" associates with their surface. Although the drivers of corona formation are not known, it is widely accepted that this layer mediates biological interactions of the nanocarrier with its surroundings. Label-free optical methods can be used to study protein corona formation without interfering with its dynamics. We demonstrate the proof-ofconcept for a multi-parametric surface plasmon resonance (MP-SPR) technique in monitoring the formation of a protein corona on surface-immobilized liposomes subjected to flowing 100 % human serum. We observed the formation of formulation-dependent "hard" and "soft" coronas with distinct refractive indices, layer thicknesses, and surface mass densities. MP-SPR was also employed to determine the affinity (K-D) of a complement system molecule (C3b) with cationic liposomes with and without polyethylene glycol. Tendency to create a thick corona correlated with a higher affinity of opsonin C3b for the surface. The label-free platform provides a fast and robust preclinical tool for tuning nanocarrier surface architecture and composition to control protein corona formation.
Subject: 317 Pharmacy
Peer reviewed: Yes
Rights: unspecified
Usage restriction: openAccess
Self-archived version: acceptedVersion


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