Multi-parametric surface plasmon resonance platform for studying liposome-serum interactions and protein corona formation

Show full item record



Permalink

http://hdl.handle.net/10138/309640

Citation

Kari , O K , Rojalin , T , Salmaso , S , Barattin , M , Jarva , H , Meri , S , Yliperttula , M , Viitala , T & Urtti , A 2017 , ' Multi-parametric surface plasmon resonance platform for studying liposome-serum interactions and protein corona formation ' , Drug Delivery and Translational Research , vol. 7 , no. 2 , pp. 1-13 . https://doi.org/10.1007/s13346-016-0320-0

Title: Multi-parametric surface plasmon resonance platform for studying liposome-serum interactions and protein corona formation
Author: Kari, Otto K.; Rojalin, Tatu; Salmaso, Stefano; Barattin, Michela; Jarva, Hanna; Meri, Seppo; Yliperttula, Marjo; Viitala, Tapani; Urtti, Arto
Contributor: University of Helsinki, Faculty of Pharmacy
University of Helsinki, Faculty of Pharmacy
University of Helsinki, HUSLAB
University of Helsinki, Medicum
University of Helsinki, Faculty of Pharmacy
University of Helsinki, Faculty of Pharmacy
University of Helsinki, Faculty of Pharmacy
Date: 2017
Language: eng
Number of pages: 13
Belongs to series: Drug Delivery and Translational Research
ISSN: 2190-393X
URI: http://hdl.handle.net/10138/309640
Abstract: When nanocarriers are administered into the blood circulation, a complex biomolecular layer known as the “protein corona” associates with their surface. Although the drivers of corona formation are not known, it is widely accepted that this layer mediates biological interactions of the nanocarrier with its surroundings. Label-free optical methods can be used to study protein corona formation without interfering with its dynamics. We demonstrate the proof-of-concept for a multi-parametric surface plasmon resonance (MP-SPR) technique in monitoring the formation of a protein corona on surface-immobilized liposomes subjected to flowing 100 % human serum. We observed the formation of formulation-dependent “hard” and “soft” coronas with distinct refractive indices, layer thicknesses, and surface mass densities. MP-SPR was also employed to determine the affinity (KD) of a complement system molecule (C3b) with cationic liposomes with and without polyethylene glycol. Tendency to create a thick corona correlated with a higher affinity of opsonin C3b for the surface. The label-free platform provides a fast and robust preclinical tool for tuning nanocarrier surface architecture and composition to control protein corona formation.
Subject: 317 Pharmacy
Rights:


Files in this item

Total number of downloads: Loading...

Files Size Format View
Kari_et_al_DDTR_2017_Revised_Manuscript_and_SI.pdf 1.039Mb PDF View/Open

This item appears in the following Collection(s)

Show full item record