CHARACTERISATION AND IN VITRO AND IN VIVO EVALUATION OF SUPERCRITICAL-CO2-FOAMED B-TCP/PLCL COMPOSITES FOR BONE APPLICATIONS

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Pitkänen , S , Paakinaho , K , Pihlman , H , Ahola , N , Hannula , M , Asikainen , S , Manninen , M , Morelius , M , Keränen , P , Hyttinen , J , Kellomäki , M , Laitinen-Vapaavuori , O & Miettinen , S 2019 , ' CHARACTERISATION AND IN VITRO AND IN VIVO EVALUATION OF SUPERCRITICAL-CO 2 -FOAMED B-TCP/PLCL COMPOSITES FOR BONE APPLICATIONS ' , European Cells & Materials , vol. 38 , pp. 35-50 . https://doi.org/10.22203/eCM.v038a04

Title: CHARACTERISATION AND IN VITRO AND IN VIVO EVALUATION OF SUPERCRITICAL-CO2-FOAMED B-TCP/PLCL COMPOSITES FOR BONE APPLICATIONS
Author: Pitkänen, S.; Paakinaho, K.; Pihlman, H.; Ahola, N.; Hannula, M.; Asikainen, S.; Manninen, M.; Morelius, M.; Keränen, P.; Hyttinen, J.; Kellomäki, M.; Laitinen-Vapaavuori, O.; Miettinen, S.
Contributor organization: Equine and Small Animal Medicine
Helsinki One Health (HOH)
Outi Vapaavuori / Principal Investigator
Softis - pehmytkudossairaudet
Petbone – ortopedia, fysioterapia, kivunlievitys
Date: 2019
Language: eng
Number of pages: 16
Belongs to series: European Cells & Materials
ISSN: 1473-2262
DOI: https://doi.org/10.22203/eCM.v038a04
URI: http://hdl.handle.net/10138/309688
Abstract: Most synthetic bone grafts are either hard and brittle ceramics or paste-like materials that differ in applicability from the gold standard autologous bone graft, which restricts their widespread use. Therefore, the aim of the study was to develop an elastic, highly porous and biodegradable beta-tricalciumphosphate/poly(L-lactide-co-epsilon-caprolactone) (beta-TCP/PLCL) composite for bone applications using supercritical CO2 foaming. Ability to support osteogenic differentiation was tested in human adipose stem cell (hASC) culture for 21 d. Biocompatibility was evaluated for 24 weeks in a rabbit femur-defect model. Foamed composites had a high ceramic content (50 wt%) and porosity (65-67 %). After 50 % compression, in an aqueous environment at 37 degrees C, tested samples returned to 95 % of their original height. Hydrolytic degradation of beta-TCP/PLCL composite, during the 24-week follow-up, was very similar to that of porous PLCL scaffold both in vitro and in vivo. Osteogenic differentiation of hASCs was demonstrated by alkaline phosphatase activity analysis, alizarin red staining, soluble collagen analysis, immunocytochemical staining and qRT-PCR. In vitro, hASCs formed a pronounced mineralised collagen matrix. A rabbit femur defect model confirmed biocompatibility of the composite. According to histological Masson-Goldner's trichrome staining and micro-computed tomography, beta-TCP/PLCL composite did not elicit infection, formation of fibrous capsule or cysts. Finally, native bone tissue at 4 weeks was already able to grow on and in the beta-TCP/PLCL composite. The elastic and highly porous beta-TCP/PLCL composite is a promising bone substitute because it is osteoconductive and easy-to-use and mould intraoperatively.
Subject: Bone substitute
composite
adipose stem cells
osteogenic differentiation
rabbit distal femur defect
beta-tricalciumphosphate
poly(L-lactide-co-epsilon-caprolactone)
ADIPOSE STEM-CELLS
POLY-L-LACTIDE
OSTEOGENIC DIFFERENTIATION
STROMAL CELLS
TISSUE
SCAFFOLDS
DEGRADATION
REGENERATION
ENHANCE
1182 Biochemistry, cell and molecular biology
216 Materials engineering
318 Medical biotechnology
413 Veterinary science
Peer reviewed: Yes
Rights: cc_by_sa
Usage restriction: openAccess
Self-archived version: publishedVersion


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