The use of human papillomavirus DNA methylation in cervical intraepithelial neoplasia : A systematic review and meta-analysis

Show full item record



Permalink

http://hdl.handle.net/10138/309753

Citation

Bowden , S J , Kalliala , I , Veroniki , A A , Arbyn , M , Mitra , A , Lathouras , K , Mirabello , L , Chadeau-Hyam , M , Paraskevaidis , E , Flanagan , J M & Kyrgiou , M 2019 , ' The use of human papillomavirus DNA methylation in cervical intraepithelial neoplasia : A systematic review and meta-analysis ' , EBioMedicine , vol. 50 , pp. 246-259 . https://doi.org/10.1016/j.ebiom.2019.10.053

Title: The use of human papillomavirus DNA methylation in cervical intraepithelial neoplasia : A systematic review and meta-analysis
Author: Bowden, Sarah J.; Kalliala, Ilkka; Veroniki, Areti A.; Arbyn, Marc; Mitra, Anita; Lathouras, Kostas; Mirabello, Lisa; Chadeau-Hyam, Marc; Paraskevaidis, Evangelos; Flanagan, James M.; Kyrgiou, Maria
Contributor organization: Clinicum
Department of Obstetrics and Gynecology
HUS Gynecology and Obstetrics
University of Helsinki
Date: 2019-12
Language: eng
Number of pages: 14
Belongs to series: EBioMedicine
ISSN: 2352-3964
DOI: https://doi.org/10.1016/j.ebiom.2019.10.053
URI: http://hdl.handle.net/10138/309753
Abstract: Background: Methylation of viral DNA has been proposed as a novel biomarker for triage of human papillomavirus (HPV) positive women at screening. This systematic review and meta-analysis aims to assess how methylation levels change with disease severity and to determine diagnostic test accuracy (DTA) in detecting high-grade cervical intra-epithelial neoplasia (CIN). Methods: We performed searches in MEDLINE, EMBASE and CENTRAL from inception to October 2019. Studies were eligible if they explored HPV methylation levels in HPV positive women. Data were extracted in duplicate and requested from authors where necessary. Random-effects models and a bivariate mixed-effects binary regression model were applied to determine pooled effect estimates. Findings: 44 studies with 8819 high-risk HPV positive women were eligible. The pooled estimates for positive methylation rate in HPV16 L1 gene were higher for high-grade CIN (>= CIN2/high-grade squamous intra-epithelial lesion (HSIL) (95% confidence interval (95%CI:72.7% (47 8-92.2))) vs. low-grade CIN (= CIN2/HSIL vs. = CIN2/HSIL vs. Interpretation: Higher HPV methylation is associated with increased disease severity, whilst HPV16 L1/L2 genes demonstrated high diagnostic accuracy to detect high-grade CIN in HPV16 positive women. Direct clinical use is limited by the need for a multi-genotype and standardised assays. Next-generation multiplex HPV sequencing assays are under development and allow potential for rapid, automated and low-cost methylation testing. (C) 2019 The Authors. Published by Elsevier B.V.
Subject: Human papillomavirus
DNA methylation
Cervical intraepithelial neoplasia
Cervical screening
Meta-analysis
E2 BINDING-SITES
RESOLUTION MELTING ANALYSIS
HPV 16 METHYLATION
L1 GENE
QUANTITATIVE MEASUREMENT
POTENTIAL BIOMARKER
CPG METHYLATION
MESSENGER-RNA
TYPE-16 DNA
E6 GENE
3121 General medicine, internal medicine and other clinical medicine
3123 Gynaecology and paediatrics
Peer reviewed: Yes
Rights: cc_by
Usage restriction: openAccess
Self-archived version: publishedVersion


Files in this item

Total number of downloads: Loading...

Files Size Format View
1_s2.0_S2352396419307285_main.pdf 2.556Mb PDF View/Open

This item appears in the following Collection(s)

Show full item record